Rat IL-6 ELISA Kit

Cat# ELR-IL6-1

Size : 1PlateKit

Brand : RayBiotech

Contact local distributor :


Phone : +1 850 650 7790

Rat IL-6 ELISA Kit

RayBio® Rat IL-6 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Contact Us for Bulk Request

Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesRat
Sensitivity
30 pg/ml

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Detection Range
30 pg/ml - 10000 pg/ml
Specificity
This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Recommended Dilution (Serum/Plasma)
2 fold
Accession Number
P20607
Gene Id
24498
Gene Symbols
IL6, IL-6
Protein Name / Synonyms
Interleukin-6 (IL-6)
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Research AreaCardiovascular Disease, Cancer, Inflammation
Shipping TypeBlue ice
Storage≤-20°C
Sample typeCell Culture Supernatants / Plasma / Serum, Cell and Tissue Lysates

Target Background

Interleukin 6 (IL-6) is a pleiotropic cytokine that regulates cell growth and differentiation of various tissues, and plays an important role in the immune response and acute phase reactions. IL-6 acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate an immune response, e.g., during infection and after trauma, especially burns or other tissue damage leading to inflammation. IL-6's role as an anti-inflammatory cytokine is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1ra and IL-10.

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

citations

  • Melatonin promotes gut anti-oxidative status in perinatal rat by remodeling the gut microbiome

    Species:
    Rat
    Sample Type:
    Serum
    Year:
    2023
    Journal Name:
    Redox biology
  • Disease-modifying effects of a glial-targeted inducible nitric oxide synthase inhibitor (1400W) in mixed-sex cohorts of a rat soman (GD) model of epilepsy

    Species:
    Rat
    Sample Type:
    Serum, cerebrospinal fluid (CSF)
    Year:
    2023
    Journal Name:
    Journal of Neuroinflammation
  • Neat1 lncRNA organizes the inflammatory gene expressions in the dorsal root ganglion in neuropathic pain caused by nerve injury

    Species:
    Rat
    Sample Type:
    spinal cord
    Year:
    2023
    Journal Name:
    Frontiers in immunology
  • Endothelial glycocalyx injury is involved in heatstroke-associated coagulopathy and protected by N-acetylcysteine

    Species:
    Rat
    Sample Type:
    Plasma
    Year:
    2023
    Journal Name:
    Frontiers in immunology
  • Myocyte-Derived Hsp90 Modulates Collagen Upregulation via Biphasic Activation of STAT-3 in Fibroblasts during Cardiac Hypertrophy

    Species:
    Rat
    Sample Type:
    myocytes
    Year:
    2023
    Journal Name:
    Molecular and Cellular Biology
  • Dapagliflozin modulates neuronal injury via instigation of LKB1/p-AMPK/GABAB R2 signaling pathway and suppression of the inflammatory cascade in an essential tremor rat model

    Species:
    Rat
    Sample Type:
    cerebella
    Year:
    2023
    Journal Name:
    Expert Opinion On Therapeutic Targets
  • Solasodine Containing Solanum torvum L. Fruit Extract Prevents Chronic Constriction Injury-Induced Neuropathic Pain in Rats: In Silico and In Vivo Evidence of TRPV1 Receptor and Cytokine Inhibition

    Species:
    Rat
    Sample Type:
    sciatic nerve
    Year:
    2023
    Journal Name:
    Molecular Neurobiology
  • Metoclopramide nanoparticles modulate immune response in a diabetic rat model: association with regulatory T cells and proinflammatory cytokines

    Species:
    Rat
    Sample Type:
    Plasma
    Year:
    2023
    Journal Name:
    International Journal of Nanomedicine
  • Air pollution combined with high-fat feeding aggravates metabolic and cardiovascular diseases: A dangerous, oxidative, and immune-inflammatory association

    Species:
    Rat
    Sample Type:
    Serum
    Year:
    2023
    Journal Name:
    LIFE SCIENCES
  • The potential of esculin in ameliorating Type-2 diabetes mellitus induced neuropathy in Wistar rats and probing its inhibitory mechanism of insulin aggregation

    Species:
    Rat
    Sample Type:
    brain
    Year:
    2023
    Journal Name:
    International Journal of Biological Macromolecules

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