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> Recombinant Human Claudin-18.2 (CLDN18.2)-VLPs (Active) - Mammalian cell

Recombinant Human Claudin-18.2 (CLDN18.2)-VLPs (Active) - Mammalian cell

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Cat# CSB-MP005498HU(A5)-20ug

Size : 20ug

Brand : Cusabio

Contact local distributor :


Phone : +1 850 650 7790

CSB-MP005498HU(A5)
Abbreviation Recombinant Human CLDN18.2 protein-VLPs (Active)
MSDS
Image
  • Western Blot
    CSB-MP005498HU(A5) is detected by Mouse anti-6*His monoclonal antibody.The three bands respectively correspond to monomer, Homodimer, Homotrimer
  • CSB-MP005498HU(A5)-AC1
    Activity
    Measured by its binding ability in a functional ELISA. Immobilized human CLDN18.2 (CSB-MP005498HU(A5)) at 5 μg/ml can bind anti-CLDN18.2 recombinant Monoclonal Antibody (CSB-RA005498A1HU), the the EC50 is 5.225-9.256 ng/ml.
  • The presence of VLP-like structures was confirmed by TEM
  • CSB-MP005498HU(A5)-WB2
    Western Blot
    CSB-MP005498HU(A5) is detected by Anti-Claudin 18 Antibody.The two bands respectively correspond to monomer, Homodimer

Product Details

Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized human CLDN18.2 (CSB-MP005498HU(A5)) at 5 μg/ml can bind anti-CLDN18.2 recombinant Monoclonal Antibody (CSB-RA005498A1HU), the the EC50 is 5.225-9.256 ng/ml.
Target Names
CLDN18.2
Uniprot No.
Alternative Names
CLDN18; UNQ778/PRO1572; Claudin-18
Molecular Characterization
Species
Homo sapiens (Human)
Source
Mammalian cell
Expression Region
1-261aa
Target Protein Sequence
MAVTACQGLGFVVSLIGIAGIIAATCMDQWSTQDLYNNPVTAVFNYQGLWRSCVRESSGFTECRGYFTLLGLPAMLQAVRALMIVGIVLGAIGLLVSIFALKCIRIGSMEDSAKANMTLTSGIMFIVSGLCAIAGVSVFANMLVTNFWMSTANMYTGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLAPEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKIYDGGARTEDEVQSYPSKHDYV
Mol. Weight
29.1 kDa
Protein Length
Full Length of Isoform A2
Tag Info
C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
Form
Lyophilized powder
Note: We will default ship it in lyophilized form with normal bule ice packs. However, if you request to ship in liquid form, it needs to be shipped with dry ice, please communicate with us in advance and extra fees for dry ice and dry ice box will be charged.
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Datasheet & COA
Please contact us to get it.
Description

Claudin-18.2 has emerged as a high-priority therapeutic target in oncology due to its restricted expression pattern and tumor-specific accessibility, yet its four-transmembrane topology makes recombinant production notoriously difficult. This virus-like particle (VLP) format presents full-length human CLDN18.2 (aa 1–261, isoform A2) in a membrane-embedded conformation produced in mammalian cells, preserving native folding of the extracellular loops critical for antibody recognition. Functional ELISA confirms that immobilized VLPs bind an anti-CLDN18.2 recombinant monoclonal...

Customer Reviews and Q&A

■ Customer Reviews
Average Rating:
5.0 - 1 reviews

Applications : Drug related studies

Review: I used the CSB-MP005498HU (A5) product for an ELISA experiment, and the EC50 was measured to be 7.918-13.26 ng/ml. The protein showed good binding activity. This product is a popular target protein used as a predictive biomarker for gastric cancer and is a commonly used protein in our compan...

By Anonymous

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