Recombinant Human Glycoprotein A33 overexpression cell lysate, derived from the transfected HEK293 cells. Purchase will also include one vial of normal control HEK293 cell lysate (Catalog # 230-10006) transfected with empty expression vector.
Recombinant Human Glycoprotein A33
Recombinant Human Glycoprotein A33
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Product Description
Specifications
| Size | 100 µg |
| Species | Human |
| Expression System | HEK293 cells |
| Tag | N-terminal 6x histidine tag |
| Purity | Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4°C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control. |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water. |
| Shipping Type | Blue ice |
| Storage | -20°C |
Protein Name
Glycoprotein A33
Synonyms
Glycoprotein A33 (Transmembrane), Transmembrane Glycoprotein A33, Transmembrane Glycoprotein A33, A33
Species
Human
Source
Expression System
Human Embryonic Kidney 293 (HEK293) Cells
Gene Symbol
GPA33
Uniprot Accession #
Expressed Region
Ile22-Val235
Conjugation/Tag
N-terminal 6x histidine tag
Molecular Mass (kDa)
Recombinant Human Glycoprotein A33 has a calculated molecular mass of 24 kDa. The actual molecular weight may increase slightly due to the potential post-modifications (PTMs).
Preparation
Purification
Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4°C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control.
Purity
Purity determined by
Specifications
Format
Lyophilized powder
Formulation
Lyophilized from a 0.2 ?m filtered solution in PBS containing mammalian cell protease inhibitor cocktail
Concentration
Determined by Pierce BCA protein assay kit
Preservatives
None
Recommended Applications
Western blotting control, antibody validation (i.e., hybridoma screening, antibody pair screening), ELISA, EIA, dot blotting, immunoprecipitation (IP), protein array, protein functional assay, protein-protein interaction, post-translational modifications, etc.
Stability & Storage
Reconstitution
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water.
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.