Recombinant human S100 calcium-binding protein A8 and A9 (S100A8-S100A9 fusion protein) overexpression cell lysate, derived from the transfected HEK293 cells. Purchase will also include one vial of normal control (Catalog #. 230-10006), the cell lysate of HEK293 cells transfected with empty expression vector.
Recombinant Human S100 Calcium-binding Protein A8 and A9 (S100A8-S100A9 fusion protein), Transfected HEK293 Cell Lysate
Recombinant Human S100 Calcium-binding Protein A8 and A9 (S100A8-S100A9 fusion protein), Transfected HEK293 Cell Lysate
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Product Description
Specifications
| Size | 100 µg |
| Species | Human |
| Accession Number | P06702|P05109 |
| Gene Symbols | S100A9, S100A8 |
| Expressed Region | S100A8: Leu2-Glu93, S100A9: Thr2-Pro114 |
| Expression System | HEK293 cells |
| Tag | C-terminal his-tag |
| Purity | Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4°C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control. |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 25. The actual molecular weight may increase slightly due to the potential post-translational modifications (PTMs). |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS (pH 7.4) containing mammalian cell protease inhibitor cocktail |
| Concentration (lot specific) | Determined by BCA protein assay |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Stability & Storage
Shipping
Including one vial of HEK293 cell lysate transfected with empty expression vector.
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.