Recombinant human S100 calcium-binding protein A8 and A9 (S100A8-S100A9 fusion protein ) overexpression cell culture supernatant, secreted from the serum-free medium of transfected HEK293 cells. Purchase will also include one vial of normal control (Catalog #. 230-20001), the culture supernatant of HEK293 cell transfected with empty expression vector.
Recombinant Human S100 Calcium-binding Protein A8 and A9 (S100A8-S100A9 Fusion Protein), Transfected HEK293 Cell Culture Supernatant
Recombinant Human S100 Calcium-binding Protein A8 and A9 (S100A8-S100A9 Fusion Protein), Transfected HEK293 Cell Culture Supernatant
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Product Description
Specifications
| Size | 200 µL |
| Species | Human |
| Accession Number | P06702|P05109 |
| Gene Symbols | S100A9, S100A8 |
| Expressed Region | S100A8: Leu2-Glu93, S100A9: Thr2-Pro114 |
| Expression System | HEK293 cells |
| Tag | C-terminal his-tag |
| Purity | Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80°C immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control. |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 25. The actual molecular weight may increase slightly due to the potential post-translational modifications (PTMs). |
| Format | Pink liquid |
| Formulation | Cell culture supernatant of transfected HEK293 cells, serum-free. |
| Shipping Type | Blue ice |
| Storage | -20°C |
Stability & Storage
Shipping
Including one vial of cell culture supernatant of HEK293 cells transfected with empty expression vector.
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.