Recombinant Human TNF-alpha
Recombinant Human TNF alpha Protein
Recombinant Human TNF alpha Protein
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg |
| Species | Human |
| Accession Number | P01375 |
| Gene Id | 7124 |
| Gene Symbols | TNFA |
| Protein Name / Synonyms | TNFa, TNF-alpha, Tumor necrosis factor |
| Expressed Region | Val77-Leu233 |
| Expression System | E. coli |
| Tag | C-terminal 6x histidine tag |
| Purity | > 95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | 17 kDa |
| Format | Liquid |
| Formulation | Supplied as a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Lot specific (see the label on the vial) |
| Preservative | None |
| Endotoxin Level | Not determined |
| Research Area | Cardiovascular Disease, Cancer, Inflammation, Antibody Drug Development, Drug Antibody (DA) Detection, Protein Therapeutics, Receptor-Ligand Binding |
| Shipping Type | Blue ice |
| Storage | -20°C |
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SDS-PAGE Image
Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant TNFR1 can bind TNFa with EC50 at 3.82 ng/mL.
Storage/Stability
Upon arrival, the protein may be stored for two weeks at 2 to 8°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA) and store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
Data Images
Figure 1
Transcription factor activity assay of NF-κB p65 from nuclear extracts of HeLa cells or HeLa cells treated with TNF-α (RayBio Cat# 230-00243). After stimulation with TNF-α, activated NF-κB p65 is translocated into the nucleus where it binds with its corresponding DNA. A. Western blotting result of NF-κB p65 from cytoplasm and nuclear fractions; B. Transcription factor activity assay of NF-κB p65 from nuclear fractions, measured by TF-ELISA kit (RayBio Cat# TFEH-p65).
Figure 2
Transcription factor activity assay of NF-κB p65 from nuclear extracts of HeLa cells or HeLa cells treated with TNF-α (RayBio Cat# 230-00243) with the specific competitor or non-specific competitor. The result shows specific binding of NF-κB p65 to the NF-κB DNA binding site.
Figure 3
Transcription factor activity assay of NF-κB p65 from nuclear extracts of HeLa cells treated with TNF-α (RayBio Cat# 230-00243) and purified NF-κB p50. No cross-reactivity has been observed between NF-κB p65 and p50.
documents
citations
Neuroinflammation, autoinflammation, splenomegaly and anemia caused by bi-allelic mutations in IRAK4
- Year:
- 2023
- Journal Name:
- Frontiers in immunology
Sanguinarine Inhibition of TNF-α-Induced CCL2, IKBKE/NF-κB/ERK1/2 Signaling Pathway, and Cell Migration in Human Triple-Negative Breast Cancer Cells
- Year:
- 2022
- Journal Name:
- International Journal of Molecular Sciences
Diallyl disulfide inhibits TNF induced CCL2 release through MAPK/ERK and NF-Kappa-B signaling
- Species:
- Human
- Year:
- 2015
- Journal Name:
- Cytokine
- PubMed ID:
- 26100848
Attenuative Effect of Diallyl Trisulfide on Caspase Activity in TNF-α-induced Triple Negative Breast Cancer Cells
- Year:
- 2023
- Journal Name:
- Anticancer Research
Diallyl Disulfide Inhibits TNF-induced CCL2 Release by MDA-MB-231 Cells
- Species:
- Human
- Sample Type:
- Conditioned Media (MDA-MB-231 Cells)
- Year:
- 2014
Development and validation of a homogeneous mobility shift assay for the measurement of infliximab and antibodies-to-infliximab levels in patient serum
- Species:
- Human
- Year:
- 2012
- Journal Name:
- Journal of Immunological Methods
- PubMed ID:
- 22691619