RHO siRNA (Human)
Cat# MBS828797-15nmol
Size : 15nmol
Brand : MyBiosource
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Phone : +1 850 650 7790
RHO siRNA – Rhodopsin (Human)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS828797MBS828797 is an siRNA targeting RHO. Also known as RHO siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit RHO expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
RHO, siRNA
Full Product Name
RHO siRNA (Human)
Product Gene Name
RHO sirna
Product Synonym Names
OPN2; Rhodopsin; Opsin-2
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
RHO siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
38,893 Da
OMIM
180380
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RHO gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for RHO sirna
RNA Interference (RNAi)
Other Notes
Small volumes of RHO sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for RHO. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
rhodopsin
NCBI Official Synonym Full Names
rhodopsin
NCBI Official Symbol
RHO
NCBI Official Synonym Symbols
RP4; OPN2; CSNBAD1
UniProt Protein Name
Rhodopsin
UniProt Synonym Protein Names
Opsin-2
Protein Family
Protein rhomboid
UniProt Gene Name
RHO
UniProt Synonym Gene Names
OPN2
UniProt Entry Name
OPSD_HUMAN
NCBI Protein Information
rhodopsin
NCBI Summary for RHO
Retinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form,which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin. This is the transmembrane protein which, when photoexcited, initiates the visual transduction cascade. Defects in this gene are also one of the causes of congenital stationary night blindness. [provided by RefSeq, Jul 2008]
Show more UniProt Comments for RHO
Rhodopsin: a G-protein coupled receptor. The light-absorbing visual pigment in Rod photoreceptor cells. Mediates vision in dim light. Consists of the apoprotein, opsin, covalently linked to cis-retinal. Defects in RHO are a cause of autosomal retinitis pigmentosa and congenital stationary night blindness.
Protein type: Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral
Chromosomal Location of Human Ortholog: 3q21-q24
Cellular Component: Golgi membrane; Golgi apparatus; photoreceptor outer segment; photoreceptor inner segment; integral to plasma membrane; plasma membrane; rough endoplasmic reticulum membrane; intercellular junction
Molecular Function: G-protein coupled receptor activity; protein binding; spectrin binding; metal ion binding; photoreceptor activity; retinal binding
Biological Process: rhodopsin mediated signaling; phototransduction, visible light; G-protein coupled receptor protein signaling pathway; red, far-red light phototransduction; regulation of rhodopsin mediated signaling; visual perception; retina development in camera-type eye; organelle organization and biogenesis; retinoid metabolic process; protein-chromophore linkage; protein amino acid phosphorylation
Disease: Fundus Albipunctatus; Night Blindness, Congenital Stationary, Autosomal Dominant 1; Retinitis Pigmentosa 4
Show more Protein type: Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral
Chromosomal Location of Human Ortholog: 3q21-q24
Cellular Component: Golgi membrane; Golgi apparatus; photoreceptor outer segment; photoreceptor inner segment; integral to plasma membrane; plasma membrane; rough endoplasmic reticulum membrane; intercellular junction
Molecular Function: G-protein coupled receptor activity; protein binding; spectrin binding; metal ion binding; photoreceptor activity; retinal binding
Biological Process: rhodopsin mediated signaling; phototransduction, visible light; G-protein coupled receptor protein signaling pathway; red, far-red light phototransduction; regulation of rhodopsin mediated signaling; visual perception; retina development in camera-type eye; organelle organization and biogenesis; retinoid metabolic process; protein-chromophore linkage; protein amino acid phosphorylation
Disease: Fundus Albipunctatus; Night Blindness, Congenital Stationary, Autosomal Dominant 1; Retinitis Pigmentosa 4
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with RHO sirna
| Reference Product | PubMed Publications |
|---|---|
| GRK1 sirna | >17 publications with RHO and GRK1 |
| GNAT1 sirna | >12 publications with RHO and GNAT1 |
| SAG sirna | >8 publications with RHO and SAG |
| RCVRN sirna | >3 publications with RHO and RCVRN |
| RLBP1 sirna | >2 publications with RHO and RLBP1 |
| GNAT2 sirna | >2 publications with RHO and GNAT2 |
| GRK7 sirna | >1 publications with RHO and GRK7 |
| GNGT1 sirna | >1 publications with RHO and GNGT1 |
Pathways associated with RHO sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| Activation Of The Phototransduction Cascade Pathway antibodies | Activation Of The Phototransduction Cascade Pathway Diagram |
| Assembly Of The Primary Cilium Pathway antibodies | Assembly Of The Primary Cilium Pathway Diagram |
| Cargo Trafficking To The Periciliary Membrane Pathway antibodies | Cargo Trafficking To The Periciliary Membrane Pathway Diagram |
| Class A/1 (Rhodopsin-like Receptors) Pathway antibodies | Class A/1 (Rhodopsin-like Receptors) Pathway Diagram |
| G Alpha (i) Signalling Events Pathway antibodies | G Alpha (i) Signalling Events Pathway Diagram |
| GPCR Downstream Signaling Pathway antibodies | GPCR Downstream Signaling Pathway Diagram |
| GPCR Ligand Binding Pathway antibodies | GPCR Ligand Binding Pathway Diagram |
| GPCRs, Class A Rhodopsin-like Pathway antibodies | GPCRs, Class A Rhodopsin-like Pathway Diagram |
| Inactivation, Recovery And Regulation Of The Phototransduction Cascade Pathway antibodies | Inactivation, Recovery And Regulation Of The Phototransduction Cascade Pathway Diagram |
| Integrated Breast Cancer Pathway antibodies | Integrated Breast Cancer Pathway Diagram |
Diseases associated with RHO sirna
| Disease Name | Pubmed Publications |
|---|---|
| Brain Diseases Antibodies | >1541 publications with RHO and Brain Diseases |
| Inflammation Antibodies | >1399 publications with RHO and Inflammation |
| Lung Diseases Antibodies | >1187 publications with RHO and Lung Diseases |
| Heart Diseases Antibodies | >1100 publications with RHO and Heart Diseases |
| Autoimmune Diseases Antibodies | >709 publications with RHO and Autoimmune Diseases |
| Pregnancy Complications Antibodies | >706 publications with RHO and Pregnancy Complications |
| Necrosis Antibodies | >695 publications with RHO and Necrosis |
| Liver Diseases Antibodies | >617 publications with RHO and Liver Diseases |
| Fibrosis Antibodies | >597 publications with RHO and Fibrosis |
| Kidney Diseases Antibodies | >533 publications with RHO and Kidney Diseases |
Organs/Tissues associated with RHO sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Muscle Antibodies | >3340 publications with RHO and Muscle |
| Brain Antibodies | >2757 publications with RHO and Brain |
| Eye Antibodies | >1218 publications with RHO and Eye |
| Uncharacterized Tissue Antibodies | >9 publications with RHO and Uncharacterized Tissue |