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> RIPA Lysis Buffer

RIPA Lysis Buffer

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Cat# R2031-75-50ml

Size : 50ml

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790

R2031-75 RIPA Lysis Buffer

Grade
Molecular Biology Grade
Applications
IP WB
Shipping Temp
RT
Storage Temp
4°C

RIPA lysis buffer is used to lyse cultured cells for immunoprecipitation and immunoblots. Similar in function to single detergent buffers that include NP-40, sodium chloride and Tris, RIPA is a good first choice for new antigens.

Solubility:
Colorless, clear, complete

pH:
7.5 ± 0.5

Components:
150mM NaCl
1.0% NP-40
0.5% deoxycholate (deoxycholic acid, sodium)
0.1% SDS
50mM Tris-Cl
Molecular Biology Grade water
0.2um sterile-filtered

Recommended Dilution:
Immediately prior to use, add the protease and phosphatase inhibitors for the following final concentrations: 1mM PMSF, 1mM sodium orthovanadate, 1mM sodium fluoride, 1ug/ml aprotinin, 1ug/ml leupeptin, 1ug/ml pepstatin

Storage and Stability:
May be stored at 4°C. Do not freeze. Stable for at 6 months after receipt. Store in the dark.

Applications
Important Note: |This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. ||Toxicity and Hazards: |All products should be handled by qualified personnel only, trained in laboratory procedures.
References
US Biological application references: 1. Zhang, Z.H. et al., (2007) Journal of Cell Science 120:4134-4143. 2. Song, K. et al., (2005) Molecular Endocrinology 19:504-515. 3. Due, M.R. et al. (2012) J. Neuroinflammation 9:200. |1. Antibodies: A Laboratory Manual, Ed Harlow, David Lane, Cold Spring Harbor Press, 449 (1988).