RS1 siRNA (Mouse)
Cat# MBS827963-15nmol
Size : 15nmol
Brand : MyBiosource
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RS1 siRNA – Retinoschisin (Mouse)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS827963MBS827963 is an siRNA targeting RS1. Also known as RS1 siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit RS1 expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
RS1, siRNA
Full Product Name
RS1 siRNA (Mouse)
Product Gene Name
RS1 sirna
Product Synonym Names
RS1H; XLRS1; Retinoschisin; X-linked juvenile retinoschisis protein homolog
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
RS1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
25,575 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RS1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for RS1 sirna
RNA Interference (RNAi)
Other Notes
Small volumes of RS1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for RS1. It may not necessarily be applicable to this product.
NCBI Official Full Name
retinoschisin
NCBI Official Synonym Full Names
retinoschisis (X-linked, juvenile) 1 (human)
NCBI Official Symbol
Rs1
NCBI Official Synonym Symbols
Rs1h; Xlrs1; tmgc1
UniProt Protein Name
Retinoschisin
UniProt Synonym Protein Names
X-linked juvenile retinoschisis protein homolog
Protein Family
Homeobox protein
UniProt Gene Name
Rs1
UniProt Synonym Gene Names
Rs1h; Xlrs1
UniProt Entry Name
XLRS1_MOUSE
NCBI Protein Information
retinoschisin
UniProt Comments for RS1
RS1: a highly conserved extracellular protein essential for proper retinal structure. Must interact with L-type voltage-gated calcium channels (L-VGCCs) correctly so that the photoreceptor cells in the eye can function properly. Genetic mutations in RS1 cause X-linked retinoschisis (XLRS) and early onset of macular degeneration. Interacts with the L-VGCCa1D subunit, regulating its activity. The expression and secretion of retinoschisin are modulated by circadian rhythms, peaking at night and diminishing during the day.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: extrinsic to plasma membrane; extracellular region
Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; phosphatidylinositol-3,4,5-triphosphate binding; phosphatidylethanolamine binding; phosphatidylserine binding; phosphatidylinositol-5-phosphate binding; phosphatidylcholine binding; phosphatidylinositol-3,4-bisphosphate binding; phosphatidylinositol 3-phosphate binding
Biological Process: adaptation of rhodopsin mediated signaling; cell adhesion
Show more Protein type: Secreted, signal peptide; Secreted
Cellular Component: extrinsic to plasma membrane; extracellular region
Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; phosphatidylinositol-3,4,5-triphosphate binding; phosphatidylethanolamine binding; phosphatidylserine binding; phosphatidylinositol-5-phosphate binding; phosphatidylcholine binding; phosphatidylinositol-3,4-bisphosphate binding; phosphatidylinositol 3-phosphate binding
Biological Process: adaptation of rhodopsin mediated signaling; cell adhesion
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with RS1 sirna
| Reference Product | PubMed Publications |
|---|---|
| Crx sirna | >5 publications with RS1 and Crx |
| Rpe65 sirna | >3 publications with RS1 and Rpe65 |
| Abca4 sirna | >2 publications with RS1 and Abca4 |
| Nr2e3 sirna | >1 publications with RS1 and Nr2e3 |
Diseases associated with RS1 sirna
| Disease Name | Pubmed Publications |
|---|---|
| Retinoschisis Antibodies | >167 publications with RS1 and Retinoschisis |
| Brain Diseases Antibodies | >10 publications with RS1 and Brain Diseases |
| Lung Diseases Antibodies | >9 publications with RS1 and Lung Diseases |
| Chromosome Aberrations Antibodies | >7 publications with RS1 and Chromosome Aberrations |
| Necrosis Antibodies | >7 publications with RS1 and Necrosis |
| Neoplasms, Experimental Antibodies | >7 publications with RS1 and Neoplasms, Experimental |
| Immune System Diseases Antibodies | >6 publications with RS1 and Immune System Diseases |
| Nerve Degeneration Antibodies | >6 publications with RS1 and Nerve Degeneration |
| Sarcoma Antibodies | >5 publications with RS1 and Sarcoma |
| Abnormalities, Multiple Antibodies | >5 publications with RS1 and Abnormalities, Multiple |
Organs/Tissues associated with RS1 sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Eye Antibodies | >174 publications with RS1 and Eye |