Rubella IgG ELISA Kit

Intended Uses: The Rubella IgG ELISA Kit is intended for the detection of IgG antibody to Rubella in humanserum or plasma.

Principle of the Assay: Diluted patient serum is added to wells coated with purified antigen. IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample.

Small volumes of Rubella IgG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS580119 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Rubella IgG, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Rubella IgG. The ELISA analytical biochemical technique of the MBS580119 kit is based on Rubella IgG antibody-Rubella IgG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Rubella IgG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Rubella IgG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).