Sambucus nigra lectin (EBL II/SNA II) isaffinity purified from elderberry bark. This lectin is a glycoprotein containing 7.8% carbohydrate and is not blood group specific. It is composed of two subunits and has a molecular weight of 140,000. EBL II has a specificity for carbohydrates containing terminal N-acetylgalactosamine and galactose residues. The blood group A-active trisaccharide containing L-fucosyl group linked alpha1-2 to galactose is 10-fold lesser as an inhibitor than the parent oligosaccharide (GalNAc alpha1-3Gal), which suggests steric hindrance to binding by the alpha-L-fucosyl group and explains the failure of the lectin to exhibit blood group A specificity. EBL II induces caspase-dependent apoptosis at low concentrations (nM) order, leading to typical symptoms of cell death in sensitive cells. This effect seems dependent on the carbohydrate binding B-chain.

Source:
Elderberry

Blood Specificity:
Non-specific

Carbohydrate Specificity:
GalNac>Galactose

Inhibitory Carbohydrate:
High concentration Lactose>beta-Galactose

Divalent Ions:
None Required

Activity:
<10ug/ml will exhibit agglutination with 3% type O human red blood cells in 10 minutes at RT. The lectin will also react with neuraminidase treated erythrocytes.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.