Sandwich ELISA
A sandwich ELISA was performed using SARS-CoV-2 N recombinant monoclonal antibody, clone 75G5a (Cat # RAB01053) as a capture antibody and SARS-CoV-2 N recombinant monoclonal antibody, clone 85C1 (Biotin) (Cat # RAB01060) as a detection antibody. SARS-CoV-2 N Protein was serially diluted 1:2 starting at 25 ng/mL. SARS-CoV-2 N recombinant monoclonal antibody, clone 75G5a (Cat # RAB01053) and SARS-CoV-2 N recombinant monoclonal antibody, clone 85C1 (Biotin) (Cat # RAB01060) detected SARS-CoV-2 N Protein antigen at very high sensitivity as low as 25 pg/mL.
Enzyme-linked Immunoabsorbent Assay
Microtiter wells were coated with N (SARS-CoV-2) Recombinant Protein (Cat # P6687) at 1 ug/mL. SARS-CoV-2 N recombinant monoclonal antibody, clone 85C1 (Biotin) (Cat # RAB01060) was serially diluted 1:2 starting at 1 ug/mL, and shows high-sensitivity binding to SARS-CoV-2 N Protein antigen.
- Specifications
Product Description
Rabbit recombinant monoclonal antibody raised against SARS-CoV-2 N.
Antibody Species
Rabbit
Immunogen
A synthetic peptide corresponding to C-terminus of SARS-CoV-2 N.
Reactivity
SARS-CoV, SARS-CoV-2
Specificity
Highly-sensitive to both SARS-CoV-2 and SARS-CoV nucleoprotein.
Form
Liquid
Conjugation
Biotin
Preparation Method
Mammalian cell (HEK293) expression system
Purification
Protein A purification
Concentration
0.5 mg/mL
Isotype
IgG
Recommend Usage
ELISA
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C.
Note
75G5a (Cat # RAB01053), 84C4a (Cat # RAB01055), 84D7 (Cat # RAB01057), 85C1 (Cat # RAB01059), 85C10 (Cat # RAB01061), 85B4 (Cat # RAB01063), and 85E9 (Cat # RAB01065) can be directly coated to the ELISA wells as a capture antibody and the other antibody (e.g. biotinylated) [75G5a (Cat # RAB01054), 84C4a (Cat # RAB01056), 84D7 (Cat # RAB01058), 85C1 (Cat # RAB01060), 85C10 (Cat # RAB01062), 85B4 (Cat # RAB01064), and 85E9 (Cat # RAB01066)] can be used as a detecting antibody with StreptAvidin-HRP. For highest sensitivity, we recommend pairing unconjugated clone 75G5a (Cat # RAB01053), immobilized on the ELISA plate to capture COVID-19 NP antigen, with SARS-CoV-2 N recombinant monoclonal antibody, clone 85C10 (Biotin) (Cat # RAB01062) for detection using high-sensitivity streptavidin-HRP (Pierce #21130).
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. - Applications
Sandwich ELISA
A sandwich ELISA was performed using SARS-CoV-2 N recombinant monoclonal antibody, clone 75G5a (Cat # RAB01053) as a capture antibody and SARS-CoV-2 N recombinant monoclonal antibody, clone 85C1 (Biotin) (Cat # RAB01060) as a detection antibody. SARS-CoV-2 N Protein was serially diluted 1:2 starting at 25 ng/mL. SARS-CoV-2 N recombinant monoclonal antibody, clone 75G5a (Cat # RAB01053) and SARS-CoV-2 N recombinant monoclonal antibody, clone 85C1 (Biotin) (Cat # RAB01060) detected SARS-CoV-2 N Protein antigen at very high sensitivity as low as 25 pg/mL.Enzyme-linked Immunoabsorbent Assay
Microtiter wells were coated with N (SARS-CoV-2) Recombinant Protein (Cat # P6687) at 1 ug/mL. SARS-CoV-2 N recombinant monoclonal antibody, clone 85C1 (Biotin) (Cat # RAB01060) was serially diluted 1:2 starting at 1 ug/mL, and shows high-sensitivity binding to SARS-CoV-2 N Protein antigen.