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> Sirtinol [410536-97-9]

Sirtinol [410536-97-9]

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Cat# HY-13515-50mg

Size : 50mg

Brand : MedChemExpress

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Phone : +1 850 650 7790

Description

Sirtinol is a sirtuin (SIRT) inhibitor, with IC50s of 48 μM, 57.7 μM and 131 μM for ySir2, hSIRT2 and hSIRT2, respectively[1][2][3][4].

IC50 & Target[4]

ySir2

48 μM (IC50)

hSIRT2

57.7 μM (IC50)

hSIRT1

131 μM (IC50)

Cellular Effect
Cell Line Type Value Description References
Fibroblast IC50
>25 μM
Compound: Sirtinol
Toxicity in neonatal foreskin fibroblasts
Toxicity in neonatal foreskin fibroblasts
[PMID: 18212103]
Hs 683 IC50
33.9 μM
Compound: 2
Antiproliferative activity against human Hs683 cells after 72 hrs by MTT assay
Antiproliferative activity against human Hs683 cells after 72 hrs by MTT assay
[PMID: 28475330]
Neutrophil IC50
>10 μM
Compound: 1
Inhibition of O2 generation in fMLP/CB-induced human neutrophils by cell based assay
Inhibition of O2 generation in fMLP/CB-induced human neutrophils by cell based assay
[PMID: 37939864]
Neutrophil IC50
1.92 μM
Compound: 1
Inhibition of elastase release in fMLP/CB-induced human neutrophils by cell based assay
Inhibition of elastase release in fMLP/CB-induced human neutrophils by cell based assay
[PMID: 37939864]
U-373MG ATCC IC50
39.3 μM
Compound: 2
Antiproliferative activity against human U373 cells after 72 hrs by MTT assay
Antiproliferative activity against human U373 cells after 72 hrs by MTT assay
[PMID: 28475330]
In Vitro

Sirtinol reduces the growth of MCF-7 cells in a concentration- and time-dependent manner. The IC50 values of sirtinol are 48.6 μM and 43.5 μM after 24 and 48 h of treatment, respectively. Sirtinol significantly decreases SIRT1 expression and increases the acetylated p53 level[1]. Sirtinol attenuates the proliferation and induces apoptosis of nonsmall cell lung cancer (NSCLC) H1299 cells and causes the significantly increased level of FoxO3a, a proapoptotic transcription factor targeted by Sirt1[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Sirtinol Related Antibodies
In Vivo

Sirtinol has anti-inflammatory effects through direct inhibition of HNE activity and attenuates HNE-induced and LPS-mediated tissue or organ injury[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

394.47

Formula

C26H22N2O2
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(NC(C1=CC=CC=C1)C)C2=CC=CC=C2/N=C/C3=C4C=CC=CC4=CC=C3O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 11.67 mg/mL (29.58 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5350 mL 12.6752 mL 25.3505 mL
5 mM 0.5070 mL 2.5350 mL 5.0701 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1 mg/mL (2.54 mM); Clear solution

    This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Purity & Documentation
References

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