SON siRNA (Human)
Cat# MBS828261-30nmol
Size : 30nmol
Brand : MyBiosource
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Phone : +1 850 650 7790
SON siRNA – Protein SON Isoform E (Human)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS828261MBS828261 is an siRNA targeting SON. Also known as SON siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit SON expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
SON, siRNA
Full Product Name
SON siRNA (Human)
Product Gene Name
SON sirna
Product Synonym Names
C21orf50; DBP5; KIAA1019; NREBP; Protein SON; Bax antagonist selected in saccharomyces 1; BASS1; Negative regulatory element-binding protein; NRE-binding protein; Protein DBP-5; SON3
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
SON siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
247,823 Da
OMIM
182465
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SON gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for SON sirna
RNA Interference (RNAi)
Other Notes
Small volumes of SON sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for SON. It may not necessarily be applicable to this product.
NCBI Official Full Name
protein SON isoform E
NCBI Official Synonym Full Names
SON DNA binding protein
NCBI Official Symbol
SON
NCBI Official Synonym Symbols
SON3; BASS1; DBP-5; NREBP; C21orf50
UniProt Protein Name
Protein SON
UniProt Synonym Protein Names
Bax antagonist selected in saccharomyces 1; BASS1; Negative regulatory element-binding protein; NRE-binding protein; Protein DBP-5; SON3
Protein Family
Protein
UniProt Gene Name
SON
UniProt Synonym Gene Names
C21orf50; DBP5; KIAA1019; NREBP; BASS1; NRE-binding protein
UniProt Entry Name
SON_HUMAN
NCBI Protein Information
protein SON
NCBI Summary for SON
This gene encodes a protein that contains multiple simple repeats. The encoded protein binds RNA and promotes pre-mRNA splicing, particularly of transcripts with poor splice sites. The protein also recognizes a specific DNA sequence found in the human hepatitis B virus (HBV) and represses HBV core promoter activity. There is a pseudogene for this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Show more UniProt Comments for SON
SON: RNA-binding protein that acts as a mRNA splicing cofactor by promoting efficient splicing of transcripts that posses weak splice sites. Specifically promotes splicing of many cell-cycle and DNA-repair transcripts that posses weak splice sites, such as TUBG1, KATNB1, TUBGCP2, AURKB, PCNT, AKT1, RAD23A, and FANCG. Probably acts by facilitating the interaction between Serine/arginine-rich proteins such as SRSF2 and the RNA polymerase II. Also binds to DNA; binds to the consensus DNA sequence: 5'- GA[GT]AN[CG][AG]CC-3'. May indirectly repress hepatitis B virus (HBV) core promoter activity and transcription of HBV genes and production of HBV virions. 10 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; RNA-binding
Chromosomal Location of Human Ortholog: 21q22.11
Cellular Component: nucleoplasm; nuclear speck
Molecular Function: protein binding; RS domain binding; DNA binding; nucleic acid binding; RNA binding
Biological Process: regulation of cell cycle; regulation of RNA splicing; RNA splicing; cytokinesis; microtubule cytoskeleton organization and biogenesis; mRNA processing; negative regulation of apoptosis
Show more Protein type: DNA-binding; RNA-binding
Chromosomal Location of Human Ortholog: 21q22.11
Cellular Component: nucleoplasm; nuclear speck
Molecular Function: protein binding; RS domain binding; DNA binding; nucleic acid binding; RNA binding
Biological Process: regulation of cell cycle; regulation of RNA splicing; RNA splicing; cytokinesis; microtubule cytoskeleton organization and biogenesis; mRNA processing; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with SON sirna
| Reference Product | PubMed Publications |
|---|---|
| UBC sirna | >2 publications with SON and UBC |
| TFPI sirna | >2 publications with SON and TFPI |
Diseases associated with SON sirna
| Disease Name | Pubmed Publications |
|---|---|
| Nervous System Diseases Antibodies | >1968 publications with SON and Nervous System Diseases |
| Brain Diseases Antibodies | >925 publications with SON and Brain Diseases |
| Heart Diseases Antibodies | >638 publications with SON and Heart Diseases |
| Inflammation Antibodies | >481 publications with SON and Inflammation |
| Liver Diseases Antibodies | >376 publications with SON and Liver Diseases |
| Disease Models, Animal Antibodies | >375 publications with SON and Disease Models, Animal |
| Kidney Diseases Antibodies | >366 publications with SON and Kidney Diseases |
| Necrosis Antibodies | >351 publications with SON and Necrosis |
| Adenocarcinoma Antibodies | >321 publications with SON and Adenocarcinoma |
| Breast Neoplasms Antibodies | >267 publications with SON and Breast Neoplasms |
Organs/Tissues associated with SON sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >2880 publications with SON and Blood |
| Brain Antibodies | >2660 publications with SON and Brain |
| Bone Antibodies | >833 publications with SON and Bone |
| Vascular Antibodies | >808 publications with SON and Vascular |
| Heart Antibodies | >726 publications with SON and Heart |
| Liver Antibodies | >706 publications with SON and Liver |
| Muscle Antibodies | >700 publications with SON and Muscle |
| Skin Antibodies | >700 publications with SON and Skin |
| Nerve Antibodies | >681 publications with SON and Nerve |
| Kidney Antibodies | >591 publications with SON and Kidney |