Excitation (blue) and emission (green) spectrum of green-fluorescent DNA stain bound to dsDNA

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
store dark

Shelf Life: 12 months

Form: liquid, supplied in 20 mM Tris-HCl pH 8.5, 0.1 mM EDTA and 0.01 % Tween-20

Color: orange

Concentration: 100 μM

Spectroscopic Properties: λ_exc 495 nm, λ_em 520 nm (bound to DNA)

Description:
Green-fluorescent DNA stain is structurally similar to SYBR® GREEN and a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling.

Green-Fluorescent DNA Stain is supplied as 100 μM concentration. Vortex Green-Fluorescent DNA Stain thoroughly prior to its use. An Green-Fluorescent DNA Stain concentration of 0.5-1.0 μM in the final assay is recommended. Add Green-Fluorescent DNA Stain as indicated in the table below per assay. Please note that the preparation of a master mix may be crucial in quantitative PCR reactions to reduce pipetting errors.
Select the optical setting for SYBR® GREEN or FAM on the detection instrument.

® SYBR is a trademark of Molecular Probes, Inc.

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