Orb1821223-100ml | SynBlock ELISA Blocking Buffer Biovalley

SynBlock ELISA Blocking Buffer

SKU: orb1821223

Description

Eliminate interference and nonspecific background associated with antibody-coated ELISA formats and sandwich ELISAs. ELISA Blocking Buffer (formerly known as Synblock) provides low background performance without the use of conventional cross-reactive protein additives.

Images & Validation

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Application Notes	1. Coat antibody or antigen onto the ELISA plate using ICT’s Antibody Coating Buffer or Antigen Coating Buffer. 2. Incubate 8–24 hours at room temperature (RT). 3. Aspirate the coating solution. 4. Wash each well twice with ICT’s ELISA Wash Buffer. 5. Block the uncoated regions of the ELISA plate by pipetting 300–400 µL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution. 6. Incubate 8–24 hours at RT. For best blocking, incubate overnight at RT. 7. Aspirate the blocking buffer; do not wash. 8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8°C.

Application Notes

1. Coat antibody or antigen onto the ELISA plate using ICT’s Antibody Coating Buffer or Antigen Coating Buffer. 2. Incubate 8–24 hours at room temperature (RT). 3. Aspirate the coating solution. 4. Wash each well twice with ICT’s ELISA Wash Buffer. 5. Block the uncoated regions of the ELISA plate by pipetting 300–400 µL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution. 6. Incubate 8–24 hours at RT. For best blocking, incubate overnight at RT. 7. Aspirate the blocking buffer; do not wash. 8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8°C.

Key Properties

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Storage & Handling

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Storage	2-8°C
Concentration	1 X
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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SynBlock ELISA Blocking Buffer [orb623167]
Sizes Available: 1 l
Block Buffer Optimization Pack [orb1409891]
Sizes Available: 5 x 100 ml
SynBlock ELISA Blocking Buffer [orb1821222]
Sizes Available: 500 ml

SynBlock ELISA Blocking Buffer

Cat# orb1821223-100ml

Contact local distributor :

Brand : Biorbyt

Contact local distributor :

SynBlock ELISA Blocking Buffer

Description

Images & Validation

Key Properties

Storage & Handling

SynBlock ELISA Blocking Buffer [orb623167]

Block Buffer Optimization Pack [orb1409891]

SynBlock ELISA Blocking Buffer [orb1821222]

Protocol Information