TNF, Recombinant, Marmota Monax, aa57-233, His-B2M-Tag (Tumor Necrosis Factor)
Cat# 375616-20ug
Size : 20ug
Brand : US Biological
Contact local distributor :
Phone : +1 850 650 7790
375616 TNF, Recombinant, Marmota Monax, aa57-233, His-B2M-Tag (Tumor Necrosis Factor)
Swiss Prot
O35734Grade
PurifiedShipping Temp
Blue IceStorage Temp
-20°CCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Source:
Recombinant protein corresponding to aa57-233 from marmota monax Tumor Necrosis Factor, fused to His-B2M-Tag at N-terminal, expressed in E. coli.
Molecular Weight:
~33.7kD
Amino Acid Sequence:
GPQREEFLNNLPLSPQAQMLTLRSSSQNMNDKPVAHVVAKNEDKEQLVWLSRRANALLANGMELIDNQLVVPANGLYLVYSQVLFKGQGCPSYVLLTHTVSRFAVSYQDKVNLLSAIKSPCPKESLEGAEFKPWYEPIYLGGVFELQKGDRLSAEVNLPSYLDFAESGQVYFGVIAL
Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.