Uncoated Mouse GFAP(glial fibrillary acidic protein) ELISA Kit

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Cat# E-UNEL-M0185-5x96T

Size : 5x96T

Contact local distributor :

Phone : +1 850 650 7790

Brand : Elabscience

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Contact local distributor :

Phone : +1 850 650 7790

Uncoated Mouse GFAP(glial fibrillary acidic protein) ELISA Kit (E-UNEL-M0185)

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Manual MSDS

Uncoated Mouse GFAP(glial fibrillary acidic protein) ELISA Kit - 1

All	Size	Price	Qty
	96T*5	$ 260.00	- +
	96T*15	$ 550.00	- +

Ancillary Products Available: Ancillary Reagent Kit

For research use only. Order now, ship in 3 days

Product Summary

The new series of Elabscience^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	24 h
Reactivity	Mouse
Specificity	This kit recognizes Mouse GFAP in samples.No significant cross-reactivity or interference between Mouse GFAP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T5 / 96T15
Storage	-20℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	Mean OD	Corrected OD
500	2.410	2.297
250	1.917	1.834
125	1.427	1.345
62.5	0.908	0.825
31.25	0.538	0.625
15.63	0.383	0.371
7.81	0.278	0.196
0	0.083	-

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GFAP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GFAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GFAP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GFAP. You can calculate the concentration of Human GFAP in the samples by comparing the OD of the samples to the standard curve.

Background

Uniport ID	P03995
Research Area	Cancer , Neuroscience , Signal Transduction , Stem Cells , Tags And CellMarkers

Cat.No.	Product Name	Sizes
E-ELIR-K001	Ancillary Reagent Kit	96T*5

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Videos

Resources

• Comprehensive Guide to Uncoated ELISA Kits: Working Principles, Benefits, Application, and Troubleshooting • ELISA Sample Processing Methods • ELISA Explained: How It Works and How to Interpret Results with Standard Curve Analysis