Vimentin (VIM) (HRP)
Cat# MBS6124568-0,1mL
Size : 0.1mL
Brand : MyBiosource
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Phone : +1 850 650 7790
Formalin-paraffin human melanoma stained with Vimentin MAb (SPM576).
Vimentin (VIM) Antibody – Mouse Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6124568MBS6124568 is an antibody targeting VIM. Also known as Vimentin (VIM) (HRP). Reactive in Bovine, Canine, Chicken, Feline, Goat, Human, Porcine. Host: Mouse. More details
Product Overview
Online Datasheet in PDFProduct Name
Vimentin, Monoclonal Antibody
Full Product Name
Vimentin (VIM) (HRP)
Product Gene Name
anti-VIM antibody
Product Synonym Names
VIM
Clonality
Monoclonal
Isotype
IgG1
Clone Number
SPM576
Host
Mouse
Species Reactivity
Bovine, Canine, Chicken, Feline, Goat, Human, Porcine
Form/Format
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with horseradish peroxidase (HRP).
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Specificity
Recognizes Vimentin. Species Crossreactivity: Human, bovine, Canine, Feline, Porcine, Goat and Chicken. Does not react with mouse and rat.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
53,652 Da
Chromosome Location
Chromosome: 10; NC_000010.10 (17270258..17279592). Location: 10p13
OMIM
193060
Immunogen
Human vimentin recombinant protein. Cellular Localization: Cytoplasmic
Conjugate
HRP
Positive Control
Jurkat cells, Sarcomas, Melanomas
Product Categories/Family for anti-VIM antibody
Antibodies; Extracellular Matrix Proteins
Applications Tested/Suitable for anti-VIM antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-VIM antibody
IP: 1-2ug/500ug protein lysate
WB: 0.5-1ug/ml
IHC: 0.5-1ug/ml for 30 minutes at RT
Applications are based on unconjugated antibody.
WB: 0.5-1ug/ml
IHC: 0.5-1ug/ml for 30 minutes at RT
Applications are based on unconjugated antibody.
Product References and Citations for anti-VIM antibody
1. Osborn M et. al. European Journal of Cell Biology. 1984; 34:137-143.
Other Notes
Small volumes of anti-VIM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for VIM. It may not necessarily be applicable to this product.
NCBI Official Full Name
vimentin
NCBI Official Synonym Full Names
vimentin
NCBI Official Symbol
VIM
UniProt Protein Name
Vimentin
Protein Family
Vimentin
UniProt Gene Name
VIM
UniProt Entry Name
VIME_HUMAN
NCBI Protein Information
vimentin
NCBI Summary for VIM
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]
Show more UniProt Comments for VIM
Vimentin: an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 10p13
Cellular Component: intermediate filament cytoskeleton; neuron projection; focal adhesion; cytoskeleton; cytoplasm; leading edge; plasma membrane; intermediate filament; peroxisome; cytosol
Molecular Function: protein C-terminus binding; identical protein binding; protein binding; structural constituent of cytoskeleton; double-stranded RNA binding; glycoprotein binding; structural constituent of eye lens
Biological Process: Bergmann glial cell differentiation; viral reproduction; apoptosis; intermediate filament organization; cell motility; astrocyte development; cell structure disassembly during apoptosis; muscle filament sliding
Disease: Cataract 30
Show more Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 10p13
Cellular Component: intermediate filament cytoskeleton; neuron projection; focal adhesion; cytoskeleton; cytoplasm; leading edge; plasma membrane; intermediate filament; peroxisome; cytosol
Molecular Function: protein C-terminus binding; identical protein binding; protein binding; structural constituent of cytoskeleton; double-stranded RNA binding; glycoprotein binding; structural constituent of eye lens
Biological Process: Bergmann glial cell differentiation; viral reproduction; apoptosis; intermediate filament organization; cell motility; astrocyte development; cell structure disassembly during apoptosis; muscle filament sliding
Disease: Cataract 30
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Diseases associated with anti-VIM antibody
| Disease Name | Pubmed Publications |
|---|---|
| Nervous System Diseases Antibodies | >383 publications with VIM and Nervous System Diseases |
| Neoplasms Antibodies | >283 publications with VIM and Neoplasms |
| Brain Diseases Antibodies | >246 publications with VIM and Brain Diseases |
| Neurodegenerative Diseases Antibodies | >166 publications with VIM and Neurodegenerative Diseases |
| Inflammation Antibodies | >46 publications with VIM and Inflammation |
| Kidney Diseases Antibodies | >37 publications with VIM and Kidney Diseases |
| Hemorrhage Antibodies | >32 publications with VIM and Hemorrhage |
| Disease Models, Animal Antibodies | >32 publications with VIM and Disease Models, Animal |
| Pain Antibodies | >31 publications with VIM and Pain |
| Liver Diseases Antibodies | >29 publications with VIM and Liver Diseases |
Organs/Tissues associated with anti-VIM antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >461 publications with VIM and Brain |
| Blood Antibodies | >187 publications with VIM and Blood |
| Muscle Antibodies | >101 publications with VIM and Muscle |
| Nerve Antibodies | >79 publications with VIM and Nerve |
| Bone Antibodies | >69 publications with VIM and Bone |
| Kidney Antibodies | >52 publications with VIM and Kidney |
| Liver Antibodies | >51 publications with VIM and Liver |
| Bone Marrow Antibodies | >46 publications with VIM and Bone Marrow |
| Eye Antibodies | >38 publications with VIM and Eye |
| Vascular Antibodies | >35 publications with VIM and Vascular |