WAC siRNA (Human)
Cat# MBS829194-30nmol
Size : 30nmol
Brand : MyBiosource
Contact local distributor :
Phone : +1 850 650 7790
WAC siRNA – Ww Domain-Containing Adapter Protein with Coiled-Coil Isoform 1 (Human)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS829194MBS829194 is an siRNA targeting WAC. Also known as WAC siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit WAC expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
WAC, siRNA
Full Product Name
WAC siRNA (Human)
Product Gene Name
WAC sirna
Product Synonym Names
KIAA1844; WW domain-containing adapter protein with coiled-coil
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
WAC siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
59,505 Da
OMIM
615049
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human WAC gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for WAC sirna
RNA Interference (RNAi)
Other Notes
Small volumes of WAC sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for WAC. It may not necessarily be applicable to this product.
NCBI Official Full Name
WW domain-containing adapter protein with coiled-coil isoform 1
NCBI Official Synonym Full Names
WW domain containing adaptor with coiled-coil
NCBI Official Symbol
WAC
NCBI Official Synonym Symbols
Wwp4; BM-016; PRO1741
UniProt Protein Name
WW domain-containing adapter protein with coiled-coil
Protein Family
Fibritin
UniProt Gene Name
WAC
UniProt Synonym Gene Names
KIAA1844
UniProt Entry Name
WAC_HUMAN
NCBI Protein Information
WW domain-containing adapter protein with coiled-coil
NCBI Summary for WAC
The protein encoded by this gene contains a WW domain, which is a protein module found in a wide range of signaling proteins. This domain mediates protein-protein interactions and binds proteins containing short linear peptide motifs that are proline-rich or contain at least one proline. This gene product shares 94% sequence identity with the WAC protein in mouse, however, its exact function is not known. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]
Show more UniProt Comments for WAC
WAC: Acts as a linker between gene transcription and histone H2B monoubiquitination at 'Lys-120' (H2BK120ub1). Interacts with the RNA polymerase II transcriptional machinery via its WW domain and with RNF20-RNF40 via its coiled coil region, thereby linking and regulating H2BK120ub1 and gene transcription. Regulates the cell-cycle checkpoint activation in response to DNA damage. Positive regulator of amino acid starvation-induced autophagy. May negatively regulate the ubiquitin proteasome pathway. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 10p12.1|10p12.1-p11.2
Cellular Component: nucleoplasm; spliceosome; nuclear speck; nucleus
Molecular Function: protein binding; chromatin binding
Biological Process: histone monoubiquitination; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of macroautophagy; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; response to DNA damage stimulus
Show more Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 10p12.1|10p12.1-p11.2
Cellular Component: nucleoplasm; spliceosome; nuclear speck; nucleus
Molecular Function: protein binding; chromatin binding
Biological Process: histone monoubiquitination; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of macroautophagy; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; response to DNA damage stimulus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with WAC sirna
| Reference Product | PubMed Publications |
|---|---|
| RNF40 sirna | >1 publications with WAC and RNF40 |
| RNF20 sirna | >1 publications with WAC and RNF20 |
Diseases associated with WAC sirna
| Disease Name | Pubmed Publications |
|---|---|
| Mental Disorders Antibodies | >29 publications with WAC and Mental Disorders |
| Depressive Disorder Antibodies | >17 publications with WAC and Depressive Disorder |
| Pain Antibodies | >7 publications with WAC and Pain |
| Fibrosis Antibodies | >6 publications with WAC and Fibrosis |
| Brain Diseases Antibodies | >6 publications with WAC and Brain Diseases |
| Central Nervous System Diseases Antibodies | >6 publications with WAC and Central Nervous System Diseases |
| Necrosis Antibodies | >5 publications with WAC and Necrosis |
| Liver Diseases Antibodies | >4 publications with WAC and Liver Diseases |
| Kidney Diseases Antibodies | >3 publications with WAC and Kidney Diseases |
| Hypertrophy Antibodies | >2 publications with WAC and Hypertrophy |
Organs/Tissues associated with WAC sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >27 publications with WAC and Blood |
| Vascular Antibodies | >11 publications with WAC and Vascular |
| Bone Antibodies | >7 publications with WAC and Bone |
| Kidney Antibodies | >6 publications with WAC and Kidney |
| Liver Antibodies | >5 publications with WAC and Liver |
| Muscle Antibodies | >5 publications with WAC and Muscle |
| Skin Antibodies | >5 publications with WAC and Skin |
| Brain Antibodies | >5 publications with WAC and Brain |
| Lymph Antibodies | >4 publications with WAC and Lymph |
| Lymph Node Antibodies | >4 publications with WAC and Lymph Node |