YY1 siRNA (Human)
Cat# MBS829092-15nmol
Size : 15nmol
Brand : MyBiosource
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YY1 siRNA – Transcriptional Repressor Protein YY1 (Human)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS829092MBS829092 is an siRNA targeting YY1. Also known as YY1 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit YY1 expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
YY1, siRNA
Full Product Name
YY1 siRNA (Human)
Product Gene Name
YY1 sirna
Product Synonym Names
INO80S; Transcriptional repressor protein YY1; Delta transcription factor; INO80 complex subunit S; NF-E1; Yin and yang 1; YY-1
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
YY1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
44,713 Da
OMIM
600013
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human YY1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for YY1 sirna
RNA Interference (RNAi)
Other Notes
Small volumes of YY1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for YY1. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
transcriptional repressor protein YY1
NCBI Official Synonym Full Names
YY1 transcription factor
NCBI Official Symbol
YY1
NCBI Official Synonym Symbols
DELTA; NF-E1; UCRBP; INO80S; YIN-YANG-1
UniProt Protein Name
Transcriptional repressor protein YY1
UniProt Synonym Protein Names
Delta transcription factor; INO80 complex subunit S; NF-E1; Yin and yang 1; YY-1
Protein Family
Transcriptional repressor protein
UniProt Gene Name
YY1
UniProt Synonym Gene Names
INO80S; YY-1
UniProt Entry Name
TYY1_HUMAN
NCBI Protein Information
transcriptional repressor protein YY1
NCBI Summary for YY1
YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1. [provided by RefSeq, Jul 2008]
Show more UniProt Comments for YY1
YY1: a polycomb-group (Pc-G) protein essential for early mammalian development and the regulation of X-inactivation and certain imprinted genes. Required for the transactivation of TSIX by CTCF. The complex of CTCF-YY1-TSIX is a critical regulator of the X chromosome binary switch. Can function as an activator or a repressor depending on the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence.
Protein type: C2H2-type zinc finger protein; Cell development/differentiation; Transcription factor
Chromosomal Location of Human Ortholog: 14q
Cellular Component: transcription factor complex; nuclear matrix; PcG protein complex; nucleus
Molecular Function: protein binding; DNA binding; four-way junction DNA binding; zinc ion binding; RNA binding; transcription coactivator activity; SMAD binding; transcription corepressor activity; transcription factor activity
Biological Process: anterior/posterior pattern formation; regulation of transcription from RNA polymerase II promoter; RNA localization; camera-type eye morphogenesis; transcription, DNA-dependent; spermatogenesis; negative regulation of transcription from RNA polymerase II promoter; cell differentiation; chromosome organization and biogenesis; response to DNA damage stimulus; double-strand break repair via homologous recombination; response to UV-C
Show more Protein type: C2H2-type zinc finger protein; Cell development/differentiation; Transcription factor
Chromosomal Location of Human Ortholog: 14q
Cellular Component: transcription factor complex; nuclear matrix; PcG protein complex; nucleus
Molecular Function: protein binding; DNA binding; four-way junction DNA binding; zinc ion binding; RNA binding; transcription coactivator activity; SMAD binding; transcription corepressor activity; transcription factor activity
Biological Process: anterior/posterior pattern formation; regulation of transcription from RNA polymerase II promoter; RNA localization; camera-type eye morphogenesis; transcription, DNA-dependent; spermatogenesis; negative regulation of transcription from RNA polymerase II promoter; cell differentiation; chromosome organization and biogenesis; response to DNA damage stimulus; double-strand break repair via homologous recombination; response to UV-C
Research Articles on YY1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with YY1 sirna
| Reference Product | PubMed Publications |
|---|---|
| EZH2 sirna | >26 publications with YY1 and EZH2 |
| RYBP sirna | >20 publications with YY1 and RYBP |
| HDAC1 sirna | >18 publications with YY1 and HDAC1 |
| HDAC2 sirna | >13 publications with YY1 and HDAC2 |
| HDAC3 sirna | >11 publications with YY1 and HDAC3 |
| TP53 sirna | >10 publications with YY1 and TP53 |
| INO80 sirna | >7 publications with YY1 and INO80 |
| MDM2 sirna | >6 publications with YY1 and MDM2 |
| EP300 sirna | >5 publications with YY1 and EP300 |
| KAT2B sirna | >1 publications with YY1 and KAT2B |
Pathways associated with YY1 sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| Delta-Notch Signaling Pathway antibodies | Delta-Notch Signaling Pathway Diagram |
| E2F Transcription Factor Network Pathway antibodies | E2F Transcription Factor Network Pathway Diagram |
| Notch Signaling Pathway antibodies | Notch Signaling Pathway Diagram |
| Notch-mediated HES/HEY Network Pathway antibodies | Notch-mediated HES/HEY Network Pathway Diagram |
| SREBP Signalling Pathway antibodies | SREBP Signalling Pathway Diagram |
| Signaling Events Mediated By HDAC Class I Pathway antibodies | Signaling Events Mediated By HDAC Class I Pathway Diagram |
| MTOR Signaling Pathway antibodies | MTOR Signaling Pathway Diagram |
| P53 Pathway antibodies | P53 Pathway Diagram |
Diseases associated with YY1 sirna
| Disease Name | Pubmed Publications |
|---|---|
| Nervous System Diseases Antibodies | >43 publications with YY1 and Nervous System Diseases |
| Cell Transformation, Neoplastic Antibodies | >27 publications with YY1 and Cell Transformation, Neoplastic |
| Liver Diseases Antibodies | >26 publications with YY1 and Liver Diseases |
| Disease Progression Antibodies | >25 publications with YY1 and Disease Progression |
| Necrosis Antibodies | >24 publications with YY1 and Necrosis |
| Inflammation Antibodies | >19 publications with YY1 and Inflammation |
| Uterine Cervical Neoplasms Antibodies | >19 publications with YY1 and Uterine Cervical Neoplasms |
| Liver Neoplasms Antibodies | >18 publications with YY1 and Liver Neoplasms |
| Disease Models, Animal Antibodies | >17 publications with YY1 and Disease Models, Animal |
| Neoplasm Metastasis Antibodies | >16 publications with YY1 and Neoplasm Metastasis |
Organs/Tissues associated with YY1 sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >125 publications with YY1 and Blood |
| Muscle Antibodies | >98 publications with YY1 and Muscle |
| Brain Antibodies | >76 publications with YY1 and Brain |
| Liver Antibodies | >58 publications with YY1 and Liver |
| Heart Antibodies | >55 publications with YY1 and Heart |
| Lung Antibodies | >50 publications with YY1 and Lung |
| Bone Antibodies | >44 publications with YY1 and Bone |
| Embryonic Tissue Antibodies | >41 publications with YY1 and Embryonic Tissue |
| Vascular Antibodies | >41 publications with YY1 and Vascular |
| Prostate Antibodies | >30 publications with YY1 and Prostate |