Human CRP ELISA Kit

Référence ELH-CRP-1

Conditionnement : 1PlateKit

Marque : RayBiotech

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Téléphone : +1 850 650 7790

Human CRP ELISA Kit

RayBio® Human CRP (C-Reactive Protein) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

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Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesHuman
Sensitivity
34 pg/ml

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Detection Range
34 pg/ml - 25,000 pg/ml
Specificity
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, GM-CSF, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, -2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum
Recommended Dilution (Serum/Plasma)
20,000 fold
Accession Number
P02741
Gene Id
1401
Gene Symbols
CRP, PTX1
Protein Name / Synonyms
C-reactive protein [Cleaved into: C-reactive protein(1-205)]
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Research AreaCardiovascular Disease, Inflammation
Shipping TypeBlue ice
Storage≤-20°C

Target Background

C-reactive protein (CRP) belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. CRP is synthesized by the liver in response to factors released by fat cells (adipocytes).Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. CRP is used mainly as a marker of inflammation. Apart from liver failure, there are few known factors that interfere with CRP production. Measuring and charting CRP values can prove useful in determining disease progress or the effectiveness of treatments. Blood, usually collected in a serum-separating tube, is analysed in a medical laboratory or at the point of care.

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

documents

Excel (Sandwich-ELISA-Analysis-Tool.xlsm)

citations

  • Silica nanoparticles induce autophagy and endothelial dysfunction via the PI3K/Akt/mTOR signaling pathway

    Species:
    Human
    Sample Type:
    Conditioned Media (HUVEC conditioned media); human umbilical vein endothelial cells (HUVECs)
    Year:
    2023
    Journal Name:
    International Journal of Nanomedicine
  • Adipokines and Inflammatory Markers in Acute Myocardial Infarction Patients with and without Obstructive Sleep Apnea: A Comparative Analysis

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2023
    Journal Name:
    International Journal of Molecular Sciences
  • Effects of olanzapine on anhedonia in schizophrenia: mediated by complement factor H

    Species:
    Human
    Sample Type:
    Plasma
    Year:
    2023
    Journal Name:
    Frontiers in psychiatry
  • Probiotic effects on immunity and microbiome in HIV-1 discordant patients

    Species:
    Human
    Sample Type:
    Plasma
    Year:
    2022
    Journal Name:
    Frontiers in immunology
  • Predicting the diagnosis of various mental disorders in a mixed cohort using blood-based multi-protein model: a machine learning approach

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2022
    Journal Name:
    European Archives of Psychiatry and Clinical Neuroscience
  • Combined serum IL-6, C-reactive protein, and cortisol may distinguish patients with anhedonia in major depressive disorder

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2022
    Journal Name:
    Frontiers in molecular neuroscience
  • The Combination of Serum BDNF, Cortisol and IFN-Gamma Can Assist the Diagnosis of Major Depressive Disorder

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2022
    Journal Name:
    Neuropsychiatric Disease and Treatment
  • Elevated Serum Levels of IgG4 in Patients with Heart Failure with Reduced Ejection Fraction: A Prospective Controlled Study

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2022
    Journal Name:
    Biology-Basel
  • A randomized pilot trial to evaluate the benefit of the concomitant use of atorvastatin and Raltegravir on immunological markers in protease-inhibitor-treated subjects living with HIV

    Species:
    Human
    Sample Type:
    Plasma
    Year:
    2020
    Journal Name:
    Plos One
  • Age-related anabolic resistance of myofibrillar protein synthesis is exacerbated in obese inactive individuals.

    Species:
    Human
    Sample Type:
    Plasma (hormone concentration)
    Year:
    2017
    Journal Name:
    The Journal of Clinical Endocrinology and Metabolism

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