Quantibody® Human Th1/Th2 Array 1 Kit. Detects 10 Human Th1 and Th2 Cytokines. Suitable for all liquid sample types.
Human Th1/Th2 Array Q1
Human Th1/Th2 Array Q1
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 10 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | T Helper Cells, Adaptive Immunity |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
GM-CSF
| IFN-gamma
| IL-10
| IL-13
| IL-2
|
IL-4
| IL-5
| IL-6
| IL-8 (CXCL8)
| TNF alpha
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Th1/Th2 Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
Differentiated fibrocytes assume a functional mesenchymal phenotype with regenerative potential
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (fibrocytes and other cell types)
- Year:
- 2019
- Journal Name:
- Science Advances
- PubMed ID:
- 31086819
Alterations to the Gut Microbiota and Their Correlation With Inflammatory Factors in Chronic Kidney Disease
- Species:
- Human
- Sample Type:
- Serum (CKD patients and controls)
- Year:
- 2019
- Journal Name:
- Frontiers in Cellular and Infection Microbiology
- PubMed ID:
- 31245306
Isolation and Analysis of Plasma-Derived Exosomes in Patients With Glioma
- Species:
- Human
- Sample Type:
- Plasma (plasma exosomes); Extracellular Vesicles
- Year:
- 2019
- Journal Name:
- Frontiers in Oncology
- PubMed ID:
- 31380286
Breast cancer cell invasion mediated by Galpha12 signaling involves expression of interleukins-6 and -8, and matrix metalloproteinase-2
- Species:
- Human
- Sample Type:
- Conditioned Media (MDA-MB-231 cells)
- Year:
- 2014
- Journal Name:
- Journal of Molecular Signaling
Case report: type 1 diabetes in monozygotic quadruplets
- Species:
- Human
- Sample Type:
- Conditioned Media
- Year:
- 2012
- Journal Name:
- European Journal of Human Genetics
- PubMed ID:
- 22108602
Effect of treatment on systemic cytokines in head and neck squamous cell carcinoma patients
- Species:
- Human
- Sample Type:
- Serum (Head and Neck Squamous Cell Carcinoma patients. )
- Year:
- 2012
- Journal Name:
- Results in immunology
Probiotic helminth administration in relapsing-remitting multiple sclerosis: a phase 1 study.
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2011
- Journal Name:
- Multiple Sclerosis Journal
- PubMed ID:
- 21372112
Using Transcranial Direct Current Stimulation to Treat Depression in HIV-Infected Persons: The Outcomes of a Feasibility Study
- Species:
- Human
- Sample Type:
- Saliva (HIV and MDD patients); Serum (HIV and MDD patients)
- Year:
- 2012
- Journal Name:
- Frontier in Psychiatry
- PubMed ID:
- 22719732
(2015)Transection Speed and Impact on Perioperative Inflammatory Response - A Randomized Controlled Trial Comparing Stapler Hepatectomy and CUSA Resection
- Species:
- Human
- Sample Type:
- Serum (Preoperative and role of transection speed on recovery and cytokine levels.)
- Year:
- 2015
- Journal Name:
- Plos One
- PubMed ID:
- 26452162
A Chinese herbal decoction, Danggui Buxue Tang, activates extracellular signal-related kinase in cultured T-lymphocytes
- Species:
- Human
- Sample Type:
- Conditioned Media
- Year:
- 2007
- Journal Name:
- Febs Letters
- PubMed ID:
- 17923124
