Library quantifcation kits for Illumina®
Accurate quantitation of the number of molecules in a library is the most critical step in the preparation steps for next-generation sequencing (NGS) to obtain high-quality reading data. Indeed, in order to obtain reliable and quality reading data, it is necessary to use a sufficient quantity of DNA, and in particular for Illumina® sequencing. The use of insufficient DNA will result in low sequencing efficiency and DNA overabundance will increase cluster density and result in poor quality data.
Quantitative PCR is considered to be the most accurate and efficient method of quantification of NGS library.
The library quantitation kits for Illumina® sequencing contain all the components necessary for the qPCR quantification of libraries:
- Mix qPCR containing DNA polymerase, dNTPs and buffer
- Primers to amplify only the molecules that contain the two sequences of adapters
- The DNA standards (S1 to S6)
- A ROX solution to add depending on the device used. If the ROX solution is separate from the mix, it is a universal kit that can be used with all instruments.
Frequently Asked Questions (FAQ)
Where can I find kits products on the Biovalley website?
kits products are available in this section and can be sorted by product families or sub-categories, such as With standards, Without standard. You can browse the catalogue tree or use the search bar with a product name, catalogue reference, brand or supplier.
What types of products are available in the kits category?
The kits category brings together various product references, consumables, reagents, kits, instruments or laboratory solutions, depending on the relevant product family. The With standards, Without standard sub-categories allow you to find the products that meet your needs more quickly.
How can I quickly find a kits product?
To find a kits product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.
Does Biovalley stock several product codes in the kits category?
Yes, Biovalley distributes several product references in the kits category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.
Can I request a quote for kits products?
Yes, you can request a quote for kits products from the product page or by contacting Biovalley (info@biovalley.fr). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.
How can I find out if a kits product is available to order?
The availability of a kits product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Biovalley with the product code or name of the product you are looking for.
What should I do if I cannot find the kits product I am looking for?
If the kits product you are looking for does not appear in the catalogue, please contact Biovalley with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.
Are all kits products available under the same terms and conditions?
No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.