Library quantifcation kits for Illumina® with standards
Accurate quantitation of the number of molecules in a library is the most critical step in the preparation steps for next-generation sequencing (NGS) to obtain high-quality reading data. Indeed, in order to obtain reliable and quality reading data, it is necessary to use a sufficient quantity of DNA, and in particular for Illumina® sequencing. The use of insufficient DNA will result in low sequencing efficiency and DNA overabundance will increase cluster density and result in poor quality data.
Quantitative PCR is considered to be the most accurate and efficient method of quantification of NGS library.
The library quantitation kits for Illumina® sequencing contain all the components necessary for the qPCR quantification of libraries:
- Mix qPCR containing DNA polymerase, dNTPs and buffer
- Primers to amplify only the molecules that contain the two sequences of adapters
- The DNA standards (S1 to S6)
- A ROX solution to add depending on the device used. If the ROX solution is separate from the mix, it is a universal kit that can be used with all instruments.
Frequently Asked Questions (FAQ)
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Yes, Biovalley distributes several product references in the with standards category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.
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Are all with standards products available under the same terms and conditions?
No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.
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