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Reagents and instruments for immunology, cell biology and molecular biology.

Anti-ChAT (Choline Acetyltransferase) Antibody

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Cat# 316-ChATt

Size : 25uL

Contact local distributor :

Phone : +1 850 650 7790

Brand : Phosphosolutions

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Contact local distributor :

Phone : +1 850 650 7790

Anti-ChAT (Choline Acetyltransferase) Antibody

Our rabbit ChAT polyclonal antibody from PhosphoSolutions is produced in-house. It detects bat, chicken, feline, guinea pig, human, mouse, non-human primate, and rat ChAT and is neat serum. Works in ELISA, WB, IHC, IP.

Immunohistochemistry detection of ChAT in normal cerebral cortex tissue using Anti-Choline Acetyltransferase (ChAT) Antibody (Cat. 316-ChAT). Tissue was mounted and pariffin fixed. Heat induced antigen retrieval was performed using citrate buffer, heated, and then blocked. After washing, the primary antibody (Cat. 316-ChAT) was incubated with the sample at 1:100. Immunoreactivity is seeing as a staining pattern on the cell body, dendrites and axons of neurons.

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~68/70 kDa ChAT.

Immunohistochemistry detection of ChAT in normal cerebral cortex tissue using Anti-Choline Acetyltransferase (ChAT) Antibody (Cat. 316-ChAT). Tissue was mounted and pariffin fixed. Heat induced antigen retrieval was performed using citrate buffer, heated, and then blocked. After washing, the primary antibody (Cat. 316-ChAT) was incubated with the sample at 1:100. Immunoreactivity is seeing as a staining pattern on the cell body, dendrites and axons of neurons.

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Species Reactivity

Bat, Chicken, Feline, Guinea Pig, Human, Mouse, Non-Human Primate, Rat

Applications

IHC, IP, WB

Host Species

Rabbit

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Data Sheet

Trial Size

SKU: 316-ChAT

Ships: 1-2 business days

Product Details

Target

ChAT

Scientific Background

Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer's disease (AD) and Down syndrome (Holt et al. 1999). Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993). It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999).

Format

Neat serum

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, IP, WB

Host Species

Rabbit

Gene Name

CHAT

Molecular Weight

70 kDa

Immunogen

Recombinant human choline acetyltransferase.

Immunogen Species

Human

Species Reactivity

Bat, Chicken, Feline, Guinea Pig, Human, Mouse, Non-Human Primate, Rat

Antibody Registry ID

AB_2492056

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Neat serum.

Buffer

Neat serum

Dilution Ranges

WB: 1:1000
IHC: 1:100
IP: 1:200

Application Details

Antibody against ChAT for use in IHC(P), IP and WB. IP: 1:200 - immunoprecipitates 68 kDa active ChAT from brain and placenta; also precipitates a 27 kDa protein co-expressed with ChAT (lacking ChAT activity) in some cholinergic CNS neurons. IHC(P): 1:100 with no antigen retrieval using IHC-Select Detection with HRP-DAB; immunoreactivity seen on cell body, dendrites and axons (brown). Control tissue: cerebral cortex. Routinely evaluated by IHC-P on normal cerebral cortex. Reactivity with other species not confirmed by IHC. Concentration lot-specific - refer to COA.

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~70 kDa choline acetyltransferase protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Acetyl CoA choline O acetyltransferase antibody, Acetyl CoA:choline O acetyltransferase antibody, ChAT antibody, CHOACTase antibody, Choline acetylase antibody, choline acetyltransferase antibody, Choline O acetyltransferase antibody, Choline O-acetyltransferase antibody, CLAT_HUMAN antibody, CMS1A antibody, CMS1A2 antibody, EC 2.3.1.6 antibody, OTTHUMP00000019583 antibody, OTTHUMP00000019584 antibody

UniProt ID

P28329.

NCBI Gene ID

1103

Shipping

Blue Ice

Western Blot Protocol: Download

General References

Karson CN, Casanova MF, Kleinman JE, Griffin WS (1993) Choline acetyltransferase in schizophrenia. Am. J.Psychiatry 150:454-459. PMID: 8434662
Holt DJ, Herman MM, Hyde TM, Kleinman JE, Cinton CM, German DC, Hersh LB, Greybiel AM, Saper CB (1999) Evidence for a deficit in cholinergic interneurons in the striatum in schizophrenia. Neuroscience 94(1):21-31.PMID: 10613493
Baskin DS, Browning JL, Pirozzolo FJ, Korporaal S, Baskin JA, Appel SH (1999) Brain choline acetyltransferase and mental function in Alzheimer disease. Arch Neurol. 56:1121-1123. PMID: 10488813

Anti-ChAT (Choline Acetyltransferase) Antibody

Cat# 316-ChATt

Contact local distributor :

Brand : Phosphosolutions

Contact local distributor :

Anti-ChAT (Choline Acetyltransferase) Antibody

SKU: 316-ChAT

Product Details

General References

Bulk Order Anti-ChAT (Choline Acetyltransferase) Antibody