FPLC columns for native proteins
Heparins are negatively charged polydispersed linear polysaccharides which have the ability to bind a wide range of biomolecules including enzymes, serine protease inhibitors, growth factors, extracellular matrix proteins, DNA modification enzymes and hormone receptors. In this chromatography, heparin is not only an affinity ligand but also an ion exchanger with high charge density and distribution. Heparin chromatography is an adsorption chromatography in which biomolecules can be specifically and reversibly adsorbed by heparins immobilized on an insoluble support. An advantage of this chromatography is that heparin-binding proteins can be conveniently enriched using its concentration effect. This is especially important for separating low abundance proteins for the analysis in two-dimensional electrophoresis (2DE) or other proteomics approaches. Heparin chromatography is a powerful sample-pretreatment technology that has been widely used to fractionate proteins from extracts of prokaryotic organism or eukaryotic cells. As an example, the fractionation of fibroblast growth factors (FGFs) from the extract of mouse brain microvascular endothelial cells (MVEC) is now introduced to demonstrate the procedure of heparin chromatography.
Frequently Asked Questions (FAQ)
Where can I find native proteins products on the Biovalley website?
native proteins products are available in this section and can be sorted by product families or sub-categories, such as Ion exchange (IEX), Heparin. You can browse the catalogue tree or use the search bar with a product name, catalogue reference, brand or supplier.
What types of products are available in the native proteins category?
The native proteins category brings together various product references, consumables, reagents, kits, instruments or laboratory solutions, depending on the relevant product family. The Ion exchange (IEX), Heparin sub-categories allow you to find the products that meet your needs more quickly.
How can I quickly find a native proteins product?
To find a native proteins product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.
Does Biovalley stock several product codes in the native proteins category?
Yes, Biovalley distributes several product references in the native proteins category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.
Can I request a quote for native proteins products?
Yes, you can request a quote for native proteins products from the product page or by contacting Biovalley (info@biovalley.fr). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.
How can I find out if a native proteins product is available to order?
The availability of a native proteins product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Biovalley with the product code or name of the product you are looking for.
What should I do if I cannot find the native proteins product I am looking for?
If the native proteins product you are looking for does not appear in the catalogue, please contact Biovalley with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.
Are all native proteins products available under the same terms and conditions?
No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.