Insect cell culture media are formulated to support the growth, maintenance, and recombinant protein production of insect cell lines used in research and bioprocess development. Commonly used systems include Sf9, Sf21, and High Five™ cells, particularly for applications involving the baculovirus expression vector system (BEVS).
Serum-free and, in some formulations, protein-free media are widely used in insect cell culture to provide more controlled culture conditions, reduce variability associated with serum-derived components, and facilitate downstream processing. Medium composition can influence cell growth, viability, metabolism, baculovirus infection, recombinant protein productivity, and product characteristics.
Key Features
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Serum-free and protein-free formulations available for controlled insect cell culture.
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Support for commonly used insect cell lines, including Sf9, Sf21, and High Five™.
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Formulations for suspension culture and, where applicable, high-cell-density growth.
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Compatibility with baculovirus infection and recombinant protein expression.
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Suitability for research-scale protein production and bioprocess development.
Usual Uses
Insect cell culture media are commonly used for BEVS-based recombinant protein expression, baculovirus propagation, vaccine antigen and virus-like particle (VLP) research, and laboratory- to pilot-scale process development.
