Plant total RNA extraction - Spin-column

There are different types of spin-column purification material : Glass fiber, derivatized silica, or ion exchange membrane in column

Samples are lysed and passed through the membrane using centrifugal or vacuum force. Wash and elution solutions are subsequently passed through the membrane, and the sample is collected into a tube by centrifugation or vacuum.

Solid phase extraction such as using a spin-column based extraction method takes advantage of the fact that DNA binds to silica. The sample containing DNA is added to a column containing a silica gel or silica beads and chaotropic salts. The chaotropic salts disrupt the hydrogen bonding between strands and facilitate of the DNA to silica by causing the nucleic acids to become hydrophobic. This exposes the phosphate residues so they are available for adsorption. The DNA binds to the silica, while the rest of the solution is washed out using ehtanol to remove chaotropic salts and other unnecessary constituents. The DNA can then be rehydrated with aquaeous low salt solutions allowing for elution of the DNA from the beads.

The advantages of this type of methods are the convenience, the ease of use, the throughput flexibility and the ability to automate.

Frequently Asked Questions (FAQ)

How can I quickly find a spin-column product?

To find a spin-column product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.

Does Biovalley stock several product codes in the spin-column category?

Yes, Biovalley distributes several product references in the spin-column category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.

Can I request a quote for spin-column products?

Yes, you can request a quote for spin-column products from the product page or by contacting Biovalley (info@biovalley.fr). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.

How can I find out if a spin-column product is available to order?

The availability of a spin-column product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Biovalley with the product code or name of the product you are looking for.

What should I do if I cannot find the spin-column product I am looking for?

If the spin-column product you are looking for does not appear in the catalogue, please contact Biovalley with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.

Are all spin-column products available under the same terms and conditions?

No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.