PAX6 Polyclonal Antibody
Anti-PAX6 Antibody
Anti-PAX6 Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P26367 |
| Gene Id | 5080 |
| Gene Symbols | PAX6 |
| Protein Name / Synonyms | PAX6, AN, AN2, ASGD5, D11S812E, FVH1, MGDA, WAGR, paired box 6 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Research Area | Neuroscience |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a homeobox and paired domain-containing protein that binds DNA and functions as a regulator of transcription. Activity of this protein is key in the development of neural tissues, particularly the eye. This gene is regulated by multiple enhancers located up to hundreds of kilobases distant from this locus. Mutations in this gene or in the enhancer regions can cause ocular disorders such as aniridia and Peter's anomaly. Use of alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
46kDa/48kDa
Observed MW
47kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PAX6 (NP_000271.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PAX6 antibody (144-07334) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
Immunofluorescence analysis of A549 cells using PAX6 antibody (144-07334). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.