RayBio® Human Angiopoietin-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human ANGPT1 ELISA Kit
Human ANGPT1 ELISA Kit
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 30 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 30 pg/ml - 18000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, Angiopoietin 2, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 3 - 30 fold |
| Accession Number | Q15389 |
| Gene Id | 284 |
| Gene Symbols | ANGPT1, KIAA0003 |
| Protein Name / Synonyms | Angiopoietin-1 (ANG-1) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Cancer |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Human ANGPT1 qIPCR ELISA Kit
As low as $749.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
documents
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)
citations
Cardiac inducing colonies halt fibroblast activation and induce cardiac/endothelial cells to move and expand via paracrine signaling
- Species:
- Human
- Sample Type:
- cardiac
- Year:
- 2022
- Journal Name:
- Molecular Biology of The Cell
Disruption of vascular endothelial homeostasis in systemic juvenile idiopathic arthritis-associated macrophage activation syndrome: The dynamic roles of angiopoietin-1 and-2.
- Species:
- Human
- Sample Type:
- Plasma; Serum
- Year:
- 2016
- Journal Name:
- Cytokine
- PubMed ID:
- 26908294
Evaluation of angiopoietin 1 and 2, vascular endothelial growth factor, and tumor necrosis factor alpha levels in asthmatic children
- Species:
- Human
- Sample Type:
- Serum (Asthmatic Children)
- Year:
- 2014
- Journal Name:
- Allergy and Asthma Proceedings
Urinary Angiopoietin-2 Is Associated with Albuminuria in Patients with Type 2 Diabetes Mellitus
- Species:
- Human
- Sample Type:
- Serum (Type 2 Diabetes Mellitus); Urine (Type 2 Diabetes Mellitus)
- Year:
- 2014
- Journal Name:
- International Journal of Endocrinology
Everolimus immunosuppression reduces the serum expression of fibrosis markers in liver transplant recipients
- Species:
- Human
- Sample Type:
- Serum (Liver Transplant patients)
- Year:
- 2014
- Journal Name:
- World journal of transplantation
Are serum-free and xeno-free culture conditions ideal for large scale clinical grade expansion of Wharton's jelly derived mesenchymal stem cells A comparative study
- Species:
- Human
- Sample Type:
- Conditioned Media
- Year:
- 2014
Mesenchymal stromal cells from patients with myelodyplastic syndromedisplay distinct functional alterations that are modulated by lenalidomide
- Species:
- Human
- Sample Type:
- Conditioned Media (mesenchymal stromal cells)
- Year:
- 2013
- Journal Name:
- Haematologica
Mesenchymal stromal cells from patients with myelodyplastic syndrome display distinct functional alterations that are modulated by lenalidomide.
- Species:
- Human
- Sample Type:
- Conditioned Media (MSCs from myelodyplastic syndrome patients)
- Year:
- 2013
- Journal Name:
- Haematologica
- PubMed ID:
- 23716561
Pancreatic-carcinoma-cell-derived Pro-angiogenic Factors Can Induce Endothelial-cell Differentiation of a Subset of Circulating CD34+ Progenitors.
- Species:
- Human
- Sample Type:
- Conditioned Media (cell-free)
- Year:
- 2013
- Journal Name:
- Journal of Translational Medicine
- PubMed ID:
- 24341512
Relationship of ultrasonographic findings with synovial angiogenesis modulators in different forms of knee arthritides.
- Species:
- Human
- Sample Type:
- Synovial Fluid (knee arthritis patients)
- Year:
- 2013
- Journal Name:
- Rheumatology International
- PubMed ID:
- 22811011
