AAH-APOSIG-1-4 | Human Apoptosis Signaling Array C1 Biovalley

Human Apoptosis Signaling Array C1

Membrane-based antibody array detects 19 phosphorylated or cleaved human apoptosis proteins. Suitable for all liquid sample types but intended for use with cell and tissue lysates.

Product Description

Specifications

Size	2 Sample Kit, 4 Sample Kit, 8 Sample Kit
Species	Human
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-Quantitative	Semi-Quantitative
Number of Targets Detected	19
Recommended Applications	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Solid Support	Membrane
Design Principle	Sandwich-based
Method Of Detection	Chemiluminescence
Research Area	Apoptosis, HIF-1 alpha Signaling, IGF Signaling, JAK/STAT Signaling, MAPK Signaling, mTOR Signaling, PI3K-AKT Signaling, TGF-beta Signaling, p53 Signaling, NFkB Signaling, Wnt-beta-Catenin Signaling
Shipping Type	Blue ice
Storage	-20°C

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Akt (P-Ser473) (PKB (P-Ser473)) Gene Symbol: AKT1 PKB RAC Gene ID: 207 Accession #: P31749 ELISA Kit: RayBio Human/Mouse/Rat Phospho-Akt (S473) ELISA	ATM (P-Ser1981) Gene Symbol: ATM Gene ID: 472 Accession #: Q13315 ELISA Kit: RayBio Human Phospho-ATM (S1981) ELISA	BAD (P-Ser112) Gene Symbol: BAD BBC6 BCL2L8 Gene ID: 572 Accession #: Q92934 ELISA Kit: RayBio Human Phospho-BAD (S112) ELISA	Caspase-3 (Cleaved Asp175) Gene Symbol: CASP3 CPP32 Gene ID: 836 Accession #: P42574 ELISA Kit: RayBio Human/Mouse Cleaved-Caspase-3 (D175) ELISA	Caspase-7 (Cleaved Asp198) Gene Symbol: CASP7 MCH3 Gene ID: 840 Accession #: P55210
CHK1 (P-Ser296) Gene Symbol: CHEK1 CHK1 Gene ID: 1111 Accession #: O14757	CHK2 (P-Thr68) Gene Symbol: CHEK2 CDS1 CHK2 RAD53 Gene ID: 11200 Accession #: O96017 ELISA Kit: RayBio Human Phospho-Chk2 (T68) ELISA	eIF-2a (P-Ser52) Gene Symbol: EIF2A CDA02 MSTP004 MSTP089 Gene ID: 83939 Accession #: Q9BY44 ELISA Kit: RayBio Human Phospho-EIF2A (S52) ELISA	ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187) Gene Symbol: MAPK3 ERK1 PRKM3/MAPK1 ERK2 PRKM1 PRKM2 Gene ID: 5595/5594 Accession #: P27361/P28482 ELISA Kit: RayBio Human/Mouse/Rat Phospho-Erk1 (T202/Y204) + Phospho-Erk2 (Y185/Y187) and Total Erk1/2 ELISA	HSP27 (P-Ser82) Gene Symbol: HSPB1 HSP27 HSP28 Gene ID: 3315 Accession #: P04792 ELISA Kit: RayBio Human Phospho-HSP27 (S82) ELISA
IKBa (P-Ser32) Gene Symbol: NFKBIA IKBA MAD3 NFKBI Gene ID: 4792 Accession #: P25963 ELISA Kit: RayBio Human Phospho-I-kappa B alpha (S32) ELISA	JNK (P-Thr183/Tyr185) Gene Symbol: MAPK8 JNK1 PRKM8 SAPK1 SAPK1C Gene ID: 5599 Accession #: P45983 ELISA Kit: RayBio Human/Mouse/Rat Phospho-JNK (T183/Y185) ELISA	NFKBP65 (P-Ser536) Gene Symbol: RELA NFKB3 Gene ID: 5970 Accession #: Q04206 ELISA Kit: RayBio Human Phospho-NF-KB p65 (S536) ELISA	PARP1 (Cleaved Asp214/Gly215) Gene Symbol: PARP1 ADPRT PPOL Gene ID: 142 Accession #: P09874 ELISA Kit: RayBio Human Cleaved-PARP (D214/G215) ELISA	p27 (P-Thr198) Gene Symbol: PSMD9 Gene ID: 5715 Accession #: O00233 ELISA Kit: RayBio Human Phospho-P27/Kip1 (T198) ELISA
p38 (P-Thr180/Tyr182) Accession #: Q16539 ELISA Kit: Phospho-p38 alpha (T180/Y182) and Total p38 ELISA	P53 (P-Ser15) Gene Symbol: TP53 P53 Gene ID: 6198 Accession #: P23443 ELISA Kit: RayBio Human/Mouse/Rat Phospho-P53 (S15) ELISA	SMAD2 (Ser245/250/255) Gene Symbol: SMAD2 MADH2 MADR2 Gene ID: 4087 Accession #: Q15796 ELISA Kit: RayBio Human/Mouse/Rat Phospho-SMAD2 (SER245/250/255) ELISA	TAK1 (P-Ser412) Gene Symbol: MAP3K7 TAK1 Gene ID: 6885 Accession #: O43318 ELISA Kit: RayBio Human Phospho-TAK1 (Ser412) ELISA

Typical Results

HeLa cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with Staurosporine (STS) for 3 hours. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

Jurkat cells were either untreated (bottom panel) or treated (top panel) with Camptothecin(CPT) for 16 hours. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

RayBiotech's Apoptosis Signaling Antibody Array identified increased NFkB activity in anoikis-resistant cancer cells. Figure adapted from Bao et al., Cancer Res, 2018.

RayBiotech's Apoptosis Signaling Antibody Array revealed ADS024’s anti-apoptotic effects in UCSE-treated colonic epithelial cells. Figure adapted from Irwin et al., Front Microbiol, 2024.

RayBiotech's Apoptosis Signaling Antibody Array showed isoginkgetin-induced apoptosis in U87MG glioblastoma cells. Figure adapted from Oliva et al., Molecules, 2022.

RayBiotech's Apoptosis Signaling Antibody Array demonstrated apoptosis induction in HepG2 cells treated with sorghum bran extract. Figure adapted from Smolensky et al., J Med Food, 2018.

Kit Components

Human Apoptosis Signaling Antibody Array C1 Membranes
Blocking Buffer
Detection Antibody Cocktail
1,000X HRP-Anti-Rabbit IgG Concentrate
20X Wash Buffer I Concentrate
20X Wash Buffer II Concentrate
2X Cell Lysis Buffer Concentrate
Detection Buffer C
Detection Buffer D
8-Well Incubation Tray w/ Lid
Protease Inhibitor Cocktail
100x Phosphatase Inhibitor Cocktail I
Phosphatase Inhibitor Cocktail II
Plastic Sheets
Array Map Template
Manual

Additional Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Assay Procedure Summary

Block membranes
Incubate with Sample
Incubate with Detection Antibody Cocktail
Incubate with HRP-Conjugated anti-IgG
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20^°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20^°C and all other reagents undiluted at 4^°C for no more than 3 months.

documents

Datasheet (AAH-APOSIG-1.pdf)

SDS (AAH-APOSIG-1.pdf)

Excel (AAH-APOSIG-1_Analysis_Tool.xls)

citations

ADS024, a single-strain live biotherapeutic product of Bacillus velezensis alleviates dextran sulfate-mediated colitis in mice, protects human colonic epithelial cells against apoptosis, and maintains epithelial barrier function
Year:
2024
Journal Name:
Frontiers in microbiology
PubMed ID:
38268707
Interleukin‐4 receptor alpha signaling regulates monocyte homeostasis
Species:
Mouse
Sample Type:
Serum
Year:
2022
Journal Name:
FASEB JOURNAL
Isoginkgetin—A Natural Compound to Control U87MG Glioblastoma Cell Growth and Migration Activating Apoptosis and Autophagy
Species:
Human
Sample Type:
U87MG cell
Year:
2022
Journal Name:
Molecules
Ferulago angulata activates intrinsic pathway of apoptosis in MCF-7 cells associated with G1 cell cycle arrest via involvement of p21/p27
Species:
Human
Sample Type:
MCF-7 cell
Year:
2022
Journal Name:
Drug Design Development and Therapy
miR-146a-5p impairs melanoma resistance to kinase inhibitors by targeting COX2 and regulating NFkB-mediated inflammatory mediators.
Species:
Human
Sample Type:
Cell Lysate
Year:
2020
Journal Name:
Cell Communication and Signaling
Targeting RGS4 Ablates Glioblastoma Proliferation
Year:
2020
Journal Name:
International Journal of Molecular Sciences
PubMed ID:
32392739
The synergism of Clinacanthus nutans Lindau extracts with gemcitabine: downregulation of anti-apoptotic markers in squamous pancreatic ductal adenocarcinoma
Species:
Human
Sample Type:
Conditioned Media (cell culture media)
Year:
2019
Journal Name:
Bmc Complementary and Alternative Medicine
Proteolytic Release of the p75NTR Intracellular Domain by ADAM10 Promotes Metastasis and Resistance to Anoikis
Species:
Human
Sample Type:
Cell Lysate (carcinoma cells)
Year:
2018
Journal Name:
Cancer Research
PubMed ID:
29437707
High-Polyphenol Sorghum Bran Extract Inhibits Cancer Cell Growth Through ROS Induction, Cell Cycle Arrest, and Apoptosis
Species:
Human
Sample Type:
Cell Lysate (liver heptaocellular carcinoma)
Year:
2018
Journal Name:
Journal of Medicinal Food
PubMed ID:
29733262

Human Apoptosis Signaling Array C1

Cat# AAH-APOSIG-1-4

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Brand : RayBiotech

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Human Apoptosis Signaling Array C1