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> Recombinant Human ACE2 Protein

Recombinant Human ACE2 Protein

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Cat# 230-30165-100

Size : 100ug

Brand : RayBiotech

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Phone : +1 850 650 7790

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Recombinant Human ACE2 ProteinThis protein is used as the standard in the ELISA kit (catalog number: ELH-ACE2).Recombinant Human ACE2 Protein

Recombinant Human ACE2 Protein

Recombinant Human ACE2 Protein

Recombinant Human Angiotensin-converting Enzyme 2 (ACE2) with C-terminal His-tag, derived from the transfected human HEK293 cells.
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Product Description

Specifications

Size100 µg, 500 µg, 1000 µg
SpeciesHuman
Accession Number
Q9BYF1
Protein Name / Synonyms
ACE2, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACEH, Metalloprotease MPROT15
Expressed Region
Gln18-Ser740 (Extracellular domain)
Expression SystemHEK293 cells
TagC-terminal his-tag
Purity
>95 %
Purity Determined BySDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa)
Recombinant protein product has a calculated molecular mass of ∼83 kDa. Due to the abundant glycosylation, it migrates as approximately 90 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below.
Format
Liquid
Formulation
Supplied as a 0.2 µm filtered solution in PBS (pH 7.4)
Concentration (lot specific)
Lot specific (see the label on the vial), determined by BCA protein assay
Research AreaAngiogenesis, Inflammation, Antibody Drug Development, Drug Antibody (DA) Detection, Protein Therapeutics, Receptor-Ligand Binding
Shipping TypeBlue ice
Storage-20°C

Introduction

Angiotensin-converting enzyme 2 is a membrane-bound enzyme primarily expressed in the lungs, heart, kidneys, intestines, and vascular endothelial cells. It plays a crucial role in regulating blood pressure and fluid balance by converting angiotensin II, a vasoconstrictor, into angiotensin (1-7), which has vasodilatory and anti-inflammatory properties. In addition to its role in cardiovascular homeostasis, angiotensin-converting enzyme 2 serves as the entry receptor for SARS-CoV and SARS-CoV-2, allowing viral attachment and cell entry, making it a key focus in infectious disease research.

Reference: https://pubmed.ncbi.nlm.nih.gov/34867819/

SDS-PAGE Image
SDS-PAGE
Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with Protein Deglycosylation Kit under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.
Lane 1: Protein standard ladder (kDa)
Lane 2: Untreated protein under reducing conditions
Lane 3: Treated protein with deglycosylation enzymes under native conditions
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions
Binding Function
Binding Function
The product S1 RBD (Cat. 230-30162) was coated in 96 well plate and incubated with the serial diluted human ACE2 protein (Raybio, Cat. 230-30165). The bound ACE2 was detected by anti-ACE2 antibody using ELISA. The calculated EC50 is 4.23-9.26 ng/mL.
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

Custom Labeling Request

We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.

citations

  • The SpACE-CCM: A facile and versatile cell culture medium-based biosensor for detection of SARS-CoV-2 spike-ACE2 interaction

    Year:
    2023
    Journal Name:
    Biosensors & Bioelectronics

  • A potent and broad neutralization of SARS-CoV-2 variants of concern by DARPins

    Year:
    2022
    Journal Name:
    Nature Chemical Biology

  • The LDL receptor-related protein 1 (LRP1) facilitates ACE2-mediated endocytosis of SARS-CoV2 spike protein-containing pseudovirions

    Species:
    Human
    Sample Type:
    Purified / Recombinant Protein (recombinant protein binding assays)
    Year:
    2025
    Journal Name:
    Journal of Biological Chemistry
    PubMed ID:
    40349772

  • A tethered ligand assay to probe SARS-CoV-2:ACE2 interactions

    Year:
    2022
    Journal Name:
    Proceedings of the National Academy of Sciences of the United States of America
    PubMed ID:
    35312342

  • Directed evolution of potent neutralizing nanobodies against SARS-CoV-2 using CDR-swapping mutagenesis

    Species:
    Human
    Sample Type:
    Other / Unspecified (competitive binding with biotinylated SARS-CoV-2 RBD on yeast-displayed nanobody)
    Year:
    2021
    Journal Name:
    Cell Chemical Biology
    PubMed ID:
    34171229

  • Uptake of severe acute respiratory syndrome coronavirus 2 spike protein mediated by angiotensin converting enzyme 2 and ganglioside in human cerebrovascular cells

    Year:
    2023
    Journal Name:
    Frontiers in Neuroscience
    PubMed ID:
    36875642

  • Targeting SARS-CoV-2 by synthetic dual-acting thiol compounds that inhibit Spike/ACE2 interaction and viral protein production

    Year:
    2022
    Journal Name:
    FASEB JOURNAL

  • Common cardiac medications potently inhibit ACE2 binding to the SARS-CoV-2 Spike, and block virus penetration and infectivity in human lung cells

    Year:
    2021
    PubMed ID:
    34773067

  • "Molecular Masks" for ACE2 to Effectively and Safely Block SARS-CoV-2 Virus Entry

    Species:
    Human
    Sample Type:
    Purified / Recombinant Protein (recombinant ACE2 protein binding target)
    Year:
    2021
    Journal Name:
    International Journal of Molecular Sciences
    PubMed ID:
    34445669

  • Thermal analysis of protein stability and ligand binding in complex media

    Year:
    2022
    Journal Name:
    Thermochimica Acta

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