Ion Torrent sequencing is a sequencing method based on the detection of hydrogen ions released during DNA polymerisation. It is a sequencing method by synthesis during which a complementary strand is created from a template. Ion Torrent sequencing consists of 4 main steps: building the bank, amplification, sequencing and analysis.  Unlike Illumina sequencing, Ion Torrent or Ion Proton sequencing does not use optical signals. Rather, it exploits the fact that the addition of a dNTP to a DNA polymer releases an H+ ion. As in other types of NSNs, the input DNA or RNA is fragmented into fragments of about 200bp. Adapters are added and a molecule is placed on a bead. The molecules are amplified on the bead by emulsion PCR. Each bead is then placed in a single well of a slide.