Adhesive microscope slides, or positively charged slides, secure tissue sections during immunohistochemistry (IHC) and in situ hybridization (ISH) by providing electrostatic attraction to negatively charged cellular components. These slides prevent detachment from rigorous protocols like heat-induced epitope retrieval, washes, and staining, ensuring reliable results in pathology and research labs.

Key Benefits

• Enhanced tissue retention, even with challenging samples like bone or decalcified tissues, reducing loss during alkaline reagents or thermal processing.
• Consistent specimen positioning and orientation, preserving architectural context for accurate diagnostics.
• Minimized cross-contamination and variability, lowering repeat testing needs and improving productivity.
• Stronger adhesion under harsh IHC/ISH conditions, including multiple washes and enzymatic steps.

IHC Applications

• Maintains sections through antigen retrieval, antibody incubation, and detection for precise protein localization.
• Supports high-throughput automated staining with no peeling, ideal for cancer biomarker studies.
• Improves signal quality and reduces background in paraffin or frozen sections.

ISH Applications

• Secures tissues during probe hybridization, stringent washes, and signal amplification for mRNA/DNA detection.
• Compatible with FISH and RNAscope protocols, preventing loss in fluorescence-based assays.
• Ensures intact samples post-hybridization for reliable gene expression analysis.

Additional Advantages

• Precleaned and ready-to-use, with hydrophilic surfaces for even spreading and low autofluorescence.
• Cost-effective for bulk use in histology, cytology, and molecular pathology, with broad compatibility.