DNMT-targeting small interfering RNAs (siRNAs) are synthetic double-stranded RNA molecules (19–27 nucleotides) designed to selectively silence DNA methyltransferase (DNMT) genes, including DNMT1, DNMT3A, and DNMT3B. By promoting sequence-specific mRNA degradation, they provide an efficient approach to investigate epigenetic regulation, DNA methylation dynamics, and gene function in mammalian cells.
Key Characteristics
- High specificity: Designed for selective knockdown of individual DNMT isoforms or pooled targets.
- Reliable performance: Typically used at 10–100 nM, with maximal silencing observed 48–72 hours after transfection.
- Research quality: Supplied as highly purified duplexes with appropriate negative and positive controls.
Applications
DNMT siRNAs are widely applied in epigenetics research to investigate maintenance and de novo DNA methylation, reactivate silenced tumor suppressor genes, validate epigenetic targets, study cancer biology, and evaluate targeted RNA delivery systems for preclinical therapeutic development.